Lys444

Site Information
FkkAkRVktEDEKEK SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2407421

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 2 , 3 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 1 ) , modification-specific antibody ( 2 ) , mutation of modification site ( 2 , 3 ) , western blotting ( 2 , 3 )
Relevant cell line - cell type - tissue:	3T3 (fibroblast) ( 3 ) , HEK293T (epithelial) ( 3 ) , HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] ( 1 ) , HeLa (cervical) ( 2 )

Downstream Regulation
Effects of modification on biological processes:	cell cycle regulation ( 3 ) , transcription, inhibited ( 3 )

References
1	Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36 25218447 Curated Info
2	Colombo R, et al. (2002) The adenovirus protein Gam1 interferes with sumoylation of histone deacetylase 1. EMBO Rep 3, 1062-8 12393750 Curated Info
3	David G, Neptune MA, DePinho RA (2002) SUMO-1 modification of histone deacetylase 1 (HDAC1) modulates its biological activities. J Biol Chem 277, 23658-63 11960997 Curated Info