Ser175
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Home > Phosphorylation Site Page: > Ser175  -  CDK9 (human)

Site Information
FGLARAFsLAkNSQP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456536

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , flow cytometry ( 1 ) , immunoprecipitation ( 2 , 7 ) , mass spectrometry ( 2 , 6 , 7 , 8 ) , mutation of modification site ( 2 , 7 , 9 ) , phospho-antibody ( 1 , 3 , 4 ) , phosphopeptide mapping ( 7 ) , western blotting ( 2 , 3 , 4 )
Disease tissue studied:
leukemia ( 2 ) , T cell leukemia ( 2 )
Relevant cell line - cell type - tissue:
CD4+ (T lymphocyte) ( 1 ) , HEK293T (epithelial) ( 3 , 4 , 7 ) , HeLa (cervical) ( 9 ) , HeLa_Meta (cervical) ( 8 ) , HeLa_Pro (cervical) ( 8 ) , HeLa_Telo (cervical) ( 8 ) , Jurkat (T lymphocyte) ( 2 , 6 ) , macrophage ( 3 ) , T lymphocyte ( 1 )

Upstream Regulation
Putative in vivo kinases:
CDK7 (human) ( 2 )
Kinases, in vitro:
CDK7 (human) ( 2 )
Putative upstream phosphatases:
PPP1CA (human) ( 7 )
Phosphatases, in vitro:
PPP1CA (human) ( 7 )
Treatments:
anti-CD3/CD28 ( 5 ) , bryostatin_1 ( 1 ) , okadaic_acid ( 4 , 7 ) , phorbol_ester ( 2 , 5 ) , small_molecule_activator_of_PP1 ( 4 ) , THZ1 ( 2 )

Downstream Regulation
Effects of modification on CDK9:
enzymatic activity, induced ( 9 ) , enzymatic activity, inhibited ( 7 ) , intracellular localization ( 3 ) , molecular association, regulation ( 2 , 5 , 9 )
Effects of modification on biological processes:
transcription, altered ( 9 ) , transcription, induced ( 3 , 5 )
Induce interaction with:
BRD4 (human) ( 5 ) , CCNT1 (human) ( 7 ) , TAT (human) ( 2 )
Inhibit interaction with:
HEXIM1 (human) ( 7 )

References 

1

Pardons M, et al. (2019) Latency-Reversing Agents Induce Differential Responses in Distinct Memory CD4 T Cell Subsets in Individuals on Antiretroviral Therapy. Cell Rep 29, 2783-2795.e5
31775045   Curated Info

2

Mbonye U, et al. (2018) Cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the CDK9 activation loop promotes P-TEFb assembly with Tat and proviral HIV reactivation. J Biol Chem
29743242   Curated Info

3

Stultz RD, Cenker JJ, McDonald D (2017) Imaging HIV-1 Genomic DNA from Entry through Productive Infection. J Virol 91
28250118   Curated Info

4

Tyagi M, et al. (2015) Reactivation of latent HIV-1 provirus via targeting protein phosphatase-1. Retrovirology 12, 63
26178009   Curated Info

5

Mbonye UR, et al. (2013) Phosphorylation of CDK9 at Ser175 enhances HIV transcription and is a marker of activated P-TEFb in CD4(+) T lymphocytes. PLoS Pathog 9, e1003338
23658523   Curated Info

6

Mulhern D (2011) CST Curation Set: 13068; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]F
Curated Info

7

Ammosova T, et al. (2011) Protein Phosphatase-1 Activates CDK9 by Dephosphorylating Ser175. PLoS One 6, e18985
21533037   Curated Info

8

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

9

Yang Z, et al. (2005) Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4. Mol Cell 19, 535-45
16109377   Curated Info