Thr276
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Home > Phosphorylation Site Page: > Thr276  -  SERT (human)

Site Information
SIWKGVKtsGKVVWV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456508

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , mass spectrometry (in vitro) ( 3 ) , mutation of modification site ( 3 , 5 , 6 , 7 ) , phospho-antibody ( 2 ) , western blotting ( 2 , 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , COS (fibroblast) ( 3 ) , HeLa (cervical) ( 2 , 5 , 7 )

Upstream Regulation
Putative in vivo kinases:
PKG1 (human) ( 6 )
Kinases, in vitro:
PKG1 (human) ( 2 )
Treatments:
5-HT ( 2 ) , 8pCPT-cGMP ( 2 ) , cGMP_analog ( 2 , 6 ) , cocaine ( 2 ) , ibogaine ( 2 ) , NECA ( 2 ) , NMDG ( 2 ) , Rp-8pCPT-cGMPS ( 2 ) , Rp-cGMP ( 6 ) , SNAP ( 2 )

Downstream Regulation
Effects of modification on SERT:
activity, induced ( 1 , 5 , 6 , 7 ) , protein conformation ( 1 )

References 

1

Chan MC, Procko E, Shukla D (2022) Structural Rearrangement of the Serotonin Transporter Intracellular Gate Induced by Thr276 Phosphorylation. ACS Chem Neurosci 13, 933-945
35258286   Curated Info

2

Zhang YW, Turk BE, Rudnick G (2016) Control of serotonin transporter phosphorylation by conformational state. Proc Natl Acad Sci U S A 113, E2776-83
27140629   Curated Info

3

Sørensen L, Strømgaard K, Kristensen AS (2014) Characterization of intracellular regions in the human serotonin transporter for phosphorylation sites. ACS Chem Biol 9, 935-44
24450286   Curated Info

4

Wong A, et al. (2012) Cyclic GMP-dependent stimulation of serotonin transport does not involve direct transporter phosphorylation by cGMP-dependent protein kinase. J Biol Chem 287, 36051-8
22942288   Curated Info

5

Zhang YW, Gesmonde J, Ramamoorthy S, Rudnick G (2007) Serotonin transporter phosphorylation by cGMP-dependent protein kinase is altered by a mutation associated with obsessive compulsive disorder. J Neurosci 27, 10878-86
17913921   Curated Info

6

Ramamoorthy S, et al. (2007) Phosphorylation of threonine residue 276 is required for acute regulation of serotonin transporter by cyclic GMP. J Biol Chem 282, 11639-47
17310063   Curated Info

7

Zhang YW, Rudnick G (2005) Serotonin transporter mutations associated with obsessive-compulsive disorder and phosphorylation alter binding affinity for inhibitors. Neuropharmacology 49, 791-7
16112691   Curated Info

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