Ser383
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser383  -  STLK3 (mouse)

Site Information
VRRVPGssGHLHKTE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456506

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 10 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 2 , 6 , 11 , 12 , 15 , 16 ) , mutation of modification site ( 3 , 14 ) , phospho-antibody ( 3 , 4 , 5 , 7 , 8 , 9 , 10 , 13 ) , western blotting ( 3 , 4 , 5 , 7 , 9 , 10 , 13 )
Disease tissue studied:
melanoma skin cancer ( 16 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 , 6 , 11 ) , 'kidney, cortex' ( 8 , 9 , 10 ) , 'kidney, tubule' ( 10 ) , 'muscle, skeletal' ( 5 ) , brain ( 15 ) , kidney ( 9 , 13 ) , liver ( 12 ) , liver [leptin (mouse), homozygous knockout] ( 12 ) , oocyte ( 14 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 16 ) , spinal cord ( 4 ) , T lymphocyte ( 3 ) , testis ( 9 )

Upstream Regulation
Regulatory protein:
TRPV2 (mouse) ( 5 ) , WNK4 (mouse) ( 9 , 13 )
Putative in vivo kinases:
WNK4 (mouse) ( 14 )
Treatments:
angiotensin_2 ( 9 , 13 ) , BAPTA-AM ( 5 ) , choline ( 9 ) , high-fat diet ( 8 ) , high-potassium diet ( 9 ) , hyperosmotic shock ( 5 ) , insulin ( 11 ) , low-potassium diet ( 7 , 9 ) , low-salt diet ( 13 ) , normal chow diet ( 7 ) , SNI ( 4 ) , spironolactone ( 9 )

Downstream Regulation
Effects of modification on STLK3:
enzymatic activity, induced ( 5 , 14 ) , intracellular localization ( 7 , 10 ) , phosphorylation ( 8 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

3

Köchl R, et al. (2016) WNK1 kinase balances T cell adhesion versus migration in vivo. Nat Immunol 17, 1075-83
27400149   Curated Info

4

Kahle KT, et al. (2016) Inhibition of the kinase WNK1/HSN2 ameliorates neuropathic pain by restoring GABA inhibition. Sci Signal 9, ra32
27025876   Curated Info

5

Zanou N, et al. (2015) Osmosensation in TRPV2 dominant negative expressing skeletal muscle fibres. J Physiol 593, 3849-63
26108786   Curated Info

6

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

7

Wade JB, et al. (2015) SPAK-mediated NCC regulation in response to low-K+ diet. Am J Physiol Renal Physiol 308, F923-31
25651563   Curated Info

8

Davies M, et al. (2014) Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK. Am J Physiol Renal Physiol 307, F96-F106
24808538   Curated Info

9

Castañeda-Bueno M, et al. (2014) Modulation of NCC activity by low and high K+ intake: insights into the signaling pathways involved. Am J Physiol Renal Physiol 306, F1507-19
24761002   Curated Info

10

Vidal-Petiot E, et al. (2013) WNK1-related Familial Hyperkalemic Hypertension results from an increased expression of L-WNK1 specifically in the distal nephron. Proc Natl Acad Sci U S A 110, 14366-71
23940364   Curated Info

11

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

12

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

13

Castañeda-Bueno M, et al. (2012) Activation of the renal Na+:Cl- cotransporter by angiotensin II is a WNK4-dependent process. Proc Natl Acad Sci U S A 109, 7929-34
22550170   Curated Info

14

Gagnon KB, Delpire E (2010) On the substrate recognition and negative regulation of SPAK, a kinase modulating Na+-K+-2Cl- cotransport activity. Am J Physiol Cell Physiol 299, C614-20
20463172   Curated Info

15

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

16

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info