Ser325
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Home > Phosphorylation Site Page: > Ser325  -  OSR1 (human)

Site Information
VRRVPGssGRLHKtE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456504

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 , 5 , 7 , 10 ) , mass spectrometry (in vitro) ( 6 ) , mutation of modification site ( 1 , 12 ) , phospho-antibody ( 2 , 3 , 11 , 12 ) , western blotting ( 2 , 3 , 11 , 12 )
Disease tissue studied:
lung cancer ( 3 ) , non-small cell lung cancer ( 3 ) , non-small cell lung adenocarcinoma ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
WNK1 (human) ( 12 )
Kinases, in vitro:
WNK1 (human) ( 6 , 13 )
Treatments:
hypotonic_buffer ( 11 ) , kynurenine ( 3 ) , sorbitol ( 12 )

Downstream Regulation
Effects of modification on OSR1:
activity, induced ( 1 ) , enzymatic activity, induced ( 11 )

References 

1

Taylor CA, et al. (2018) OSR1 regulates a subset of inward rectifier potassium channels via a binding motif variant. Proc Natl Acad Sci U S A 115, 3840-3845
29581290   Curated Info

2

Conway LC, et al. (2017) N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation. J Biol Chem 292, 21253-21263
29092909   Curated Info

3

Hsu YL, et al. (2016) Lung cancer-derived galectin-1 contributes to cancer associated fibroblast-mediated cancer progression and immune suppression through TDO2/kynurenine axis. Oncotarget 7, 27584-98
27050278   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

6

Sengupta S, et al. (2013) Regulation of OSR1 and the sodium, potassium, two chloride cotransporter by convergent signals. Proc Natl Acad Sci U S A 110, 18826-31
24191005   Curated Info

7

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

8

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

9

Thastrup JO, et al. (2012) SPAK/OSR1 regulate NKCC1 and WNK activity: analysis of WNK isoform interactions and activation by T-loop trans-autophosphorylation. Biochem J 441, 325-37
22032326   Curated Info

10

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

11

Richardson C, et al. (2008) Activation of the thiazide-sensitive Na+-Cl- cotransporter by the WNK-regulated kinases SPAK and OSR1. J Cell Sci 121, 675-84
18270262   Curated Info

12

Zagórska A, et al. (2007) Regulation of activity and localization of the WNK1 protein kinase by hyperosmotic stress. J Cell Biol 176, 89-100
17190791   Curated Info

13

Vitari AC, Deak M, Morrice NA, Alessi DR (2005) The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases. Biochem J 391, 17-24
16083423   Curated Info