Ser332
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.0.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser332  -  WNK4 (mouse)

Site Information
kRAsFAKsVIGTPEF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456207

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 3 , 5 , 6 ) , mass spectrometry (in vitro) ( 2 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 6 ) , 32Dcl3 (myeloid) ( 6 ) , COS7 (fibroblast) ( 3 ) , E.coli (bacterial) ( 1 ) , HEK293T (epithelial) ( 3 ) , lung ( 5 )

Upstream Regulation
Putative upstream phosphatases:
PPP1CA (human) ( 2 )
Treatments:
angiotensin_2 ( 3 ) , bisindolylmaleimide ( 3 ) , colforsin ( 3 )

Downstream Regulation
Effects of modification on WNK4:
enzymatic activity, induced ( 1 , 3 ) , phosphorylation ( 2 )

References 

1

Schiapparelli P, et al. (2021) Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function. Cell Rep 36, 109416
34289367   Curated Info

2

Murillo-de-Ozores AR, et al. (2018) C-terminally truncated, kidney-specific variants of the WNK4 kinase lack several sites that regulate its activity. J Biol Chem
29921588   Curated Info

3

Castañeda-Bueno M, et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114, E879-E886
28096417   Curated Info

4

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

5

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

6

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.