Ser332
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser332  -  WNK4 (mouse)

Site Information
kRAsFAKsVIGTPEF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456207

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 4 , 5 ) , mass spectrometry (in vitro) ( 1 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative upstream phosphatases:
PPP1CA (human) ( 1 )
Treatments:
angiotensin_2 ( 2 ) , bisindolylmaleimide ( 2 ) , colforsin ( 2 )

Downstream Regulation
Effects of modification on WNK4:
enzymatic activity, induced ( 2 ) , phosphorylation ( 1 )

References 

1

Murillo-de-Ozores AR, et al. (2018) C-terminally truncated, kidney-specific variants of the WNK4 kinase lack several sites that regulate its activity. J Biol Chem
29921588   Curated Info

2

CastaƱeda-Bueno M, et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114, E879-E886
28096417   Curated Info

3

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

4

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

5

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info