Tyr83
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Home > Phosphorylation Site Page: > Tyr83  -  eNOS (cow)

Site Information
WELGSITyDTLCAQS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456466

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mutation of modification site ( 2 , 3 ) , phospho-antibody ( 2 , 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:
BAEC (endothelial) ( 2 ) , COS (fibroblast) ( 2 , 3 , 4 )

Upstream Regulation
Regulatory protein:
PTEN (human) ( 4 )
Putative in vivo kinases:
Src (human) ( 2 , 3 )
Kinases, in vitro:
Src (human) ( 3 )
Treatments:
angiopoietin-1 ( 2 ) , ATP ( 2 ) , bradykinin ( 2 ) , estradiol ( 2 ) , PP2 ( 2 , 3 ) , S-1-P ( 2 ) , thapsigargin ( 2 , 3 ) , vanadate ( 2 , 3 ) , VEGF ( 2 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, induced ( 2 , 3 )

References 

1

Ruan L, et al. (2013) Calcineurin-mediated dephosphorylation of eNOS at serine 116 affects eNOS enzymatic activity indirectly by facilitating c-Src binding and tyrosine 83 phosphorylation. Vascul Pharmacol 59, 27-35
23727078   Curated Info

2

Fulton D, et al. (2008) Agonist-stimulated endothelial nitric oxide synthase activation and vascular relaxation. Role of eNOS phosphorylation at Tyr83. Circ Res 102, 497-504
18096817   Curated Info

3

Fulton D, et al. (2005) Src kinase activates endothelial nitric-oxide synthase by phosphorylating Tyr-83. J Biol Chem 280, 35943-52
16123043   Curated Info

4

Church JE, et al. Inhibition of endothelial nitric oxide synthase by the lipid phosphatase PTEN. Vascul Pharmacol 52, 191-8
19962452   Curated Info