Ser722
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Home > Phosphorylation Site Page: > Ser722  -  Raptor (human)

Site Information
PrLrsVssyGNIRAV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2314601

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 11 ) , immunoassay ( 17 ) , immunoprecipitation ( 3 , 32 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 7 , 8 , 9 , 10 , 12 , 13 , 15 , 16 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 36 , 37 ) , mutation of modification site ( 3 , 11 , 32 ) , phospho-antibody ( 3 , 6 , 17 ) , western blotting ( 3 , 11 )
Disease tissue studied:
breast cancer ( 5 , 13 ) , breast ductal carcinoma ( 5 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 , 5 ) , colorectal cancer ( 6 ) , colorectal carcinoma ( 6 ) , leukemia ( 15 , 34 ) , acute myelogenous leukemia ( 15 ) , chronic myelogenous leukemia ( 34 ) , lung cancer ( 8 , 13 ) , non-small cell lung cancer ( 13 ) , non-small cell lung adenocarcinoma ( 8 ) , ovarian cancer ( 5 ) , pancreatic ductal adenocarcinoma ( 7 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 16 ) , 'pancreatic, ductal'-pancreas ( 7 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 25 ) , 293 (epithelial) [AT1 (human), transfection] ( 24 ) , 293 (epithelial) ( 3 , 20 , 28 , 32 ) , A431 (epithelial) ( 17 ) , A549 (pulmonary) ( 9 ) , breast ( 1 , 5 ) , BT-20 (breast cell) ( 13 ) , BT-549 (breast cell) ( 13 ) , C2C12 (myoblast) ( 35 ) , Calu 6 (pulmonary) ( 13 ) , CHO (fibroblast) ( 18 ) , fibroblast-embryo ( 11 ) , H2009 (pulmonary) ( 13 ) , H2077 (pulmonary) ( 13 ) , H2887 (pulmonary) ( 13 ) , H322M (pulmonary) ( 13 ) , HCC1359 (pulmonary) ( 13 ) , HCC1937 (breast cell) ( 13 ) , HCC2279 (pulmonary) ( 13 ) , HCC366 (pulmonary) ( 13 ) , HCC4006 (pulmonary) ( 13 ) , HCC78 (pulmonary) ( 13 ) , HCC827 (pulmonary) ( 13 ) , HCT116 (intestinal) ( 27 ) , HCT15 (intestinal) ( 6 ) , HEK293T (epithelial) ( 26 ) , HeLa (cervical) ( 4 , 12 , 17 , 21 , 31 , 33 , 36 ) , HeLa S3 (cervical) ( 19 ) , HOP62 (pulmonary) ( 13 ) , Jurkat (T lymphocyte) ( 10 , 22 , 23 , 29 , 30 , 37 ) , K562 (erythroid) ( 12 , 34 ) , KG-1 (myeloid) ( 15 ) , L6 (myoblast) ( 3 ) , LCLC-103H (pulmonary) ( 13 ) , LOU-NH91 (squamous) ( 13 ) , lung ( 8 ) , MCF-7 (breast cell) ( 13 ) , MDA-MB-231 (breast cell) ( 13 ) , MDA-MB-468 (breast cell) ( 13 ) , NCI-H1395 (pulmonary) ( 13 ) , NCI-H1568 (pulmonary) ( 13 ) , NCI-H157 (pulmonary) ( 13 ) , NCI-H1648 (pulmonary) ( 13 ) , NCI-H1666 (pulmonary) ( 13 ) , NCI-H2030 (pulmonary) ( 13 ) , NCI-H2172 (pulmonary) ( 13 ) , NCI-H322 (pulmonary) ( 13 ) , NCI-H460 (pulmonary) ( 13 ) , NCI-H520 (squamous) ( 13 ) , NCI-H647 (pulmonary) ( 13 ) , ovary ( 5 ) , PC9 (pulmonary) ( 13 ) , SW48 (intestinal) ( 6 ) , SW620 (intestinal) ( 6 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 35 ) , p90RSK (human) ( 32 )
Kinases, in vitro:
GSK3B (human) ( 3 ) , p90RSK (human) ( 32 )
Treatments:
BI-D1870 ( 32 ) , EGF ( 36 ) , FMK-MEA ( 6 ) , insulin ( 18 ) , ischemia ( 5 ) , PD0325901 ( 6 ) , PD98059 ( 18 ) , phorbol_ester ( 32 ) , PP242 ( 6 ) , rapamycin ( 18 ) , U0126 ( 32 ) , wortmannin ( 18 )

Downstream Regulation
Effects of modification on biological processes:
cell cycle regulation ( 17 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Stretton C, et al. (2015) GSK3-mediated raptor phosphorylation supports amino-acid-dependent mTORC1-directed signalling. Biochem J 470, 207-21
26348909   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Ducker GS, et al. (2014) Incomplete inhibition of phosphorylation of 4E-BP1 as a mechanism of primary resistance to ATP-competitive mTOR inhibitors. Oncogene 33, 1590-600
23542178   Curated Info

7

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

8

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

9

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

10

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

11

Kim SG, et al. (2013) Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Mol Cell 49, 172-85
23142078   Curated Info

12

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

13

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

14

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

15

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

16

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

17

Vazquez-Martin A, Cufí S, Oliveras-Ferraros C, Menendez JA (2011) Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle 10, 3140-52
21900751   Curated Info

18

Langlais P, Yi Z, Mandarino LJ (2011) The Identification of Raptor as a Substrate for p44/42 MAPK. Endocrinology 152, 1264-73
21325048   Curated Info

19

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

20

Carriere A, et al. (2011) ERK1/2 phosphorylate Raptor to promote Ras-dependent activation of mTOR complex 1 (mTORC1). J Biol Chem 286, 567-77
21071439   Curated Info

21

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

22

Possemato A (2010) CST Curation Set: 10731; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (81E12B5) Rabbit mAb Cat#: 10003
Curated Info

23

Possemato A (2010) CST Curation Set: 10535; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

24

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

25

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

26

Gwinn DM, Asara JM, Shaw RJ (2010) Raptor is phosphorylated by cdc2 during mitosis. PLoS One 5, e9197
20169205   Curated Info

27

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

28

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

29

Possemato A (2009) CST Curation Set: 6911; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

30

Possemato A (2009) CST Curation Set: 6744; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XX[ST]
Curated Info

31

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

32

Carrière A, et al. (2008) Oncogenic MAPK signaling stimulates mTORC1 activity by promoting RSK-mediated raptor phosphorylation. Curr Biol 18, 1269-77
18722121   Curated Info

33

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

34

Stokes M (2008) CST Curation Set: 4392; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

35

Gwinn DM, et al. (2008) AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell 30, 214-26
18439900   Curated Info

36

Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51
18220336   Curated Info

37

Stokes M (2008) CST Curation Set: 3885; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info