Ser792
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Home > Phosphorylation Site Page: > Ser792  -  Raptor (human)

Site Information
DKMRRAssYSsLNSL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2314600

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 15 ) , electrophoretic mobility shift ( 13 ) , immunoassay ( 14 ) , immunoprecipitation ( 8 ) , mass spectrometry ( 8 , 18 ) , mutation of modification site ( 8 , 13 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 8 , 10 , 11 , 12 , 14 , 15 , 16 , 17 , 19 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 8 , 10 , 11 , 12 , 13 , 15 , 16 , 17 , 19 )
Disease tissue studied:
brain cancer ( 17 ) , glioblastoma ( 17 ) , glioma ( 17 ) , breast cancer ( 12 ) , liver cancer ( 10 ) , hepatocellular carcinoma ( 10 ) , lung cancer ( 2 , 3 ) , non-small cell lung cancer ( 2 , 3 ) , non-small cell lung adenocarcinoma ( 3 ) , non-small cell large cell lung carcinoma ( 3 ) , prostate cancer ( 4 , 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
AMPKA1 (mouse) ( 19 ) , AMPKA2 (mouse) ( 19 ) , CK1E (human) ( 11 ) , HRas (human) ( 10 ) , LKB1 (human) ( 10 ) , LKB1 (mouse) ( 19 ) , PKM (human) ( 2 ) , PKM iso2 (human) ( 2 ) , SKP2 (human) ( 10 ) , ULK1 (human) ( 16 )
Putative in vivo kinases:
AMPKA1 (human) ( 20 ) , ULK1 (human) ( 15 ) , ULK2 (human) ( 15 )
Kinases, in vitro:
AMPKA1 (human) ( 20 ) , GSK3B (human) ( 8 ) , ULK1 (human) ( 15 )
Treatments:
A-769662 ( 19 ) , acadesine ( 19 ) , amino_acid_starvation ( 12 ) , amino_acids ( 12 ) , compound_C ( 13 ) , EDTA ( 19 ) , glucose ( 12 ) , glucose_starvation ( 10 , 12 , 13 , 17 ) , itraconazol ( 6 ) , KU-0063794 ( 15 ) , L-glutamine_withdrawal ( 13 ) , miR-451 ( 17 ) , pemetrexed ( 3 ) , PF-670462 ( 11 ) , phenformin ( 20 ) , resveratrol ( 20 ) , sildenafil ( 3 ) , siRNA ( 15 ) , starvation_medium ( 16 ) , triptolide ( 5 )

Downstream Regulation
Effects of modification on Raptor:
molecular association, regulation ( 15 )
Effects of modification on biological processes:
apoptosis, inhibited ( 2 ) , autophagy, induced ( 2 ) , cell cycle regulation ( 14 ) , signaling pathway regulation ( 15 )
Inhibit interaction with:
4E-BP1 (human) ( 15 )

References 

1

Zhang EB, et al. (2021) Antifungal agent Terbinafine restrains tumor growth in preclinical models of hepatocellular carcinoma via AMPK-mTOR axis. Oncogene
34247189   Curated Info

2

Prakasam G, et al. (2017) Pyruvate kinase M knockdown-induced signaling via AMP-activated protein kinase promotes mitochondrial biogenesis, autophagy, and cancer cell survival. J Biol Chem 292, 15561-15576
28778925   Curated Info

3

Booth L, et al. (2017) PDE5 inhibitors enhance the lethality of pemetrexed through inhibition of multiple chaperone proteins and via the actions of cyclic GMP and nitric oxide. Oncotarget 8, 1449-1468
27903966   Curated Info

4

Mirkheshti N, et al. (2016) Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer. Oncotarget 7, 62240-62254
27557496   Curated Info

5

Zhao F, et al. (2016) Triptolide induces protective autophagy through activation of the CaMKKβ-AMPK signaling pathway in prostate cancer cells. Oncotarget 7, 5366-82
26734992   Curated Info

6

Head SA, et al. (2015) Antifungal drug itraconazole targets VDAC1 to modulate the AMPK/mTOR signaling axis in endothelial cells. Proc Natl Acad Sci U S A 112, E7276-85
26655341   Curated Info

7

Agarwal S, Bell CM, Rothbart SB, Moran RG (2015) AMP-activated Protein Kinase (AMPK) Control of mTORC1 Is p53- and TSC2-independent in Pemetrexed-treated Carcinoma Cells. J Biol Chem 290, 27473-86
26391395   Curated Info

8

Stretton C, et al. (2015) GSK3-mediated raptor phosphorylation supports amino-acid-dependent mTORC1-directed signalling. Biochem J 470, 207-21
26348909   Curated Info

9

Ritho J, Arold ST, Yeh ET (2015) A Critical SUMO1 Modification of LKB1 Regulates AMPK Activity during Energy Stress. Cell Rep 12, 734-42
26212320   Curated Info

10

Lee SW, et al. (2015) Skp2-dependent ubiquitination and activation of LKB1 is essential for cancer cell survival under energy stress. Mol Cell 57, 1022-33
25728766   Curated Info

11

Shin S, Wolgamott L, Roux PP, Yoon SO (2014) Casein kinase 1ε promotes cell proliferation by regulating mRNA translation. Cancer Res 74, 201-11
24247720   Curated Info

12

Chen CH, et al. (2013) Autoregulation of the mechanistic target of rapamycin (mTOR) complex 2 integrity is controlled by an ATP-dependent mechanism. J Biol Chem 288, 27019-30
23928304   Curated Info

13

Kim SG, et al. (2013) Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Mol Cell 49, 172-85
23142078   Curated Info

14

Vazquez-Martin A, Cufí S, Oliveras-Ferraros C, Menendez JA (2011) Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle 10, 3140-52
21900751   Curated Info

15

Dunlop EA, et al. (2011) ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding. Autophagy 7, 737-47
21460630   Curated Info

16

Löffler AS, et al. (2011) Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop. Autophagy 7, 696-706
21460634   Curated Info

17

Godlewski J, et al. (2010) MicroRNA-451 regulates LKB1/AMPK signaling and allows adaptation to metabolic stress in glioma cells. Mol Cell 37, 620-32
20227367   Curated Info

18

Gwinn DM, Asara JM, Shaw RJ (2010) Raptor is phosphorylated by cdc2 during mitosis. PLoS One 5, e9197
20169205   Curated Info

19

Zagórska A, et al. (2010) New roles for the LKB1-NUAK pathway in controlling myosin phosphatase complexes and cell adhesion. Sci Signal 3, ra25
20354225   Curated Info

20

Gwinn DM, et al. (2008) AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell 30, 214-26
18439900   Curated Info