Thr454
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Home > Phosphorylation Site Page: > Thr454  -  PKM (human)

Site Information
RAPIIAVtRNPQtAR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 23132420

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:
esophageal cancer ( 3 ) , esophageal carcinoma ( 3 ) , liver cancer ( 1 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Pim2 (human) ( 1 )
Kinases, in vitro:
Pim2 (human) ( 1 )
Treatments:
siRNA ( 1 )

Downstream Regulation
Effects of modification on PKM:
enzymatic activity, induced ( 1 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell growth, induced ( 1 ) , transcription, induced ( 1 )

References 

1

Yu Z, et al. (2013) Proviral Insertion in Murine Lymphomas 2 (PIM2) Oncogene Phosphorylates Pyruvate Kinase M2 (PKM2) and Promotes Glycolysis in Cancer Cells. J Biol Chem 288, 35406-16
24142698   Curated Info

2

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

3

Tucker M (2010) CST Curation Set: 9227; Year: 2010; Biosample/Treatment: cell line, Kyse180/untreated; Disease: esophageal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info