Ser256
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Home > Phosphorylation Site Page: > Ser256  -  PGK1 (human)

Site Information
NNMEIGTsLFDEEGA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 23131090

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 1 ) , immunoassay ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:
brain cancer ( 1 ) , glioblastoma ( 1 ) , glioblastoma multiforme ( 1 ) , glioma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
EGFR (human) ( 1 )
Putative in vivo kinases:
CK2A1 (human) ( 1 )
Kinases, in vitro:
CK2A1 (human) ( 1 )
Treatments:
EGF ( 1 ) , TBB ( 1 )

Downstream Regulation
Effects of modification on PGK1:
molecular association, regulation ( 1 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell growth, induced ( 1 )
Induce interaction with:
CDC7 (human) ( 1 )

References 

1

Li X, et al. (2018) Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72, 650-660.e8
30392930   Curated Info

2

Mulhern D (2011) CST Curation Set: 12830; Year: 2011; Biosample/Treatment: cell line, HEK 293/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) AMPK Substrate (P-S/T(2)-102) Rabbit mAb Cat#: 5759, PTMScan(R) Phospho-AMPK Substrate Motif (LXRXXS*/T*) Immunoaffinity Beads Cat#: 5760
Curated Info

3

Mulhern D (2011) CST Curation Set: 12710; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

4

Possemato A (2010) CST Curation Set: 10167; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (60D12F4) Rabbit mAb Cat#: 10002
Curated Info