Thr31
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Home > Phosphorylation Site Page: > Thr31  -  HEC1 (human)

Site Information
VNkQGLytPQTKEkP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 23112540

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Disease tissue studied:
breast cancer ( 4 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 , 4 )
Relevant cell line - cell type - tissue:
breast ( 3 , 4 ) , HeLa (cervical) ( 1 , 2 , 7 ) , HeLa S3 (cervical) ( 6 ) , hTERT-RPE1 (epithelial) ( 2 ) , Jurkat (T lymphocyte) ( 8 )

Upstream Regulation
Regulatory protein:
BUB1B (human) ( 2 )
Putative in vivo kinases:
CDK1 (human) ( 2 )
Kinases, in vitro:
CDK1 (human) ( 2 )
Treatments:
AZ3146 ( 2 ) , AZD1152 ( 1 ) , BI2536 ( 2 ) , calyculin_A ( 2 ) , MLN8237 ( 1 , 2 ) , nocodazole ( 1 , 2 ) , okadaic_acid ( 2 ) , RO-3306 ( 2 ) , STLC ( 2 ) , TAME ( 2 ) , ZM447439 ( 1 , 2 )

Downstream Regulation
Effects of modification on biological processes:
cell cycle regulation ( 1 ) , cytoskeletal reorganization ( 2 )

References 

1

Sobajima T, et al. (2023) PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size. J Cell Biol 222
36897279   Curated Info

2

Kucharski TJ, et al. (2022) Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity. J Cell Biol 221
35878017   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

7

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

8

Possemato A (2010) CST Curation Set: 9704; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

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