Thr246
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Home > Phosphorylation Site Page: > Thr246  -  GAPDH (human)

Site Information
NVsVVDLtCrLEkPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 23109023
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 10 ) , immunoprecipitation ( 1 , 10 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 7 , 8 , 9 , 10 ) , mutation of modification site ( 6 ) , phosphopeptide mapping ( 6 ) , western blotting ( 1 )
Disease tissue studied:
lung cancer ( 2 , 3 , 4 , 5 ) , non-small cell lung cancer ( 3 , 4 , 5 ) , non-small cell lung adenocarcinoma ( 2 , 3 , 4 , 5 ) , non-small cell large cell lung carcinoma ( 3 ) , non-small cell squamous cell lung carcinoma ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 10 ) , Cal-12T (pulmonary) ( 5 ) , DMS153 (pulmonary) ( 4 ) , H9c2 (myoblast) ( 1 ) , HCC15 (pulmonary) ( 2 ) , HCC44 (pulmonary) ( 5 ) , HCC78 (pulmonary) ( 3 ) , heart ( 6 ) , HeLa (cervical) ( 7 , 9 ) , HL-1 (myocyte) ( 6 ) , Jurkat (T lymphocyte) ( 8 ) , LOU-NH91 (squamous) ( 2 ) , lung ( 2 , 3 , 4 , 5 ) , NCI-H1437 (pulmonary) ( 4 , 5 ) , NCI-H1666 (pulmonary) ( 5 ) , NCI-H1703 (squamous) ( 2 ) , NCI-H1781 (pulmonary) ( 3 ) , NCI-H2073 (pulmonary) ( 2 , 4 ) , NCI-H209 (pulmonary) ( 4 ) , NCI-H2228 (pulmonary) ( 3 ) , NCI-H2342 (pulmonary) ( 2 ) , NCI-H2405 (pulmonary) ( 5 ) , NCI-H3122 (pulmonary) ( 3 ) , NCI-H661 (pulmonary) ( 3 ) , NCI-H838 (pulmonary) ( 4 )

Upstream Regulation
Putative in vivo kinases:
PKCD (human) ( 6 )
Kinases, in vitro:
PKCD (human) ( 1 , 6 )
Treatments:
deltaV1-1 ( 6 ) , ischemia/reperfusion ( 6 )

Downstream Regulation
Effects of modification on GAPDH:
protein conformation ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 6 )

References 

1

Qvit N, et al. (2016) Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death. J Biol Chem 291, 13608-21
27129213   Curated Info

2

Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

3

Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

4

Rikova K, Hall B (2013) CST Curation Set: 20742, 21169, 30176, 30177, 30178; Year: 2013; Biosample/Treatment: cell line, H838, DMS153, H2073, H209, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

5

Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

6

Yogalingam G, Hwang S, Ferreira JC, Mochly-Rosen D (2013) Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Phosphorylation by Protein Kinase Cδ (PKCδ) Inhibits Mitochondria Elimination by Lysosomal-like Structures following Ischemia and Reoxygenation-induced Injury. J Biol Chem 288, 18947-60
23653351   Curated Info

7

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

8

Possemato A (2010) CST Curation Set: 8995; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pTXR Antibodies Used to Purify Peptides prior to LCMS: Phospho-Threonine-X-Arginine Antibody Cat#: 2351, PTMScan(R) Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads Cat#: 1988
Curated Info

9

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

10

Seo J, et al. (2008) Strategy for comprehensive identification of post-translational modifications in cellular proteins, including low abundant modifications: application to glyceraldehyde-3-phosphate dehydrogenase. J Proteome Res 7, 587-602
18183946   Curated Info

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