Ser37
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Home > Phosphorylation Site Page: > Ser37  -  CTNNB1 (human)

Site Information
yLDsGIHsGATtTAP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447582

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 51 ) , immunoprecipitation ( 4 , 5 , 10 , 16 , 36 , 38 , 39 ) , mass spectrometry ( 15 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 30 , 33 , 41 , 56 ) , mass spectrometry (in vitro) ( 36 ) , mutation of modification site ( 7 , 10 , 16 , 37 , 42 , 43 , 47 , 50 , 51 , 53 , 54 , 55 , 56 , 58 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 10 , 12 , 17 , 29 , 31 , 32 , 34 , 35 , 36 , 38 , 39 , 40 , 41 , 42 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 10 , 12 , 16 , 17 , 29 , 31 , 32 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 45 , 49 , 50 , 52 , 53 , 54 , 56 , 57 , 58 )
Disease tissue studied:
bone cancer ( 34 ) , breast cancer ( 7 , 38 , 54 ) , colorectal cancer ( 2 , 16 , 31 , 35 , 40 , 41 , 50 , 53 ) , colorectal carcinoma ( 2 , 16 , 31 , 35 , 40 , 41 , 50 , 53 ) , gastric cancer ( 15 , 39 ) , gastric carcinoma ( 15 , 39 ) , kidney cancer ( 32 ) , leukemia ( 42 ) , chronic myelogenous leukemia ( 42 ) , lung cancer ( 30 , 33 ) , non-small cell lung cancer ( 30 , 33 ) , non-small cell squamous cell lung carcinoma ( 30 ) , pancreatic cancer ( 6 ) , pancreatic carcinoma ( 6 ) , prostate cancer ( 17 ) , melanoma skin cancer ( 12 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 4 , 45 , 46 , 56 ) , 293T (epithelial) ( 16 , 31 , 40 , 53 , 54 , 55 , 58 , 59 ) , 4T1 (breast cell) ( 38 ) , A375 (melanocyte) ( 12 ) , A431 (epithelial) ( 36 ) , A498 (renal) [SFRP2 (human), transfection] ( 32 ) , AGS (gastric) ( 39 ) , BC-1 (B lymphocyte) ( 42 ) , C4-2 (prostate cell) ( 17 ) , C57MG (epithelial) ( 37 ) , Caco-2 (intestinal) ( 16 ) , Capan1 (pancreatic) ( 6 ) , CD18/HPAF (pancreatic) ( 6 ) , CHO (fibroblast) ( 58 ) , COLO-320 (intestinal) ( 2 ) , COS (fibroblast) ( 45 , 51 , 54 , 55 ) , DLD1 (intestinal) ( 40 ) , endothelial-vein ( 52 ) , FTC-133 (thyroid cell) ( 48 ) , HCT116 (intestinal) ( 31 , 41 , 53 ) , HeLa (cervical) ( 4 , 5 , 56 , 59 ) , HT-29 (intestinal) ( 44 , 53 ) , HUVEC (endothelial) ( 43 ) , Jurkat (T lymphocyte) ( 49 , 56 ) , KU812 (myeloid) ( 42 ) , L cells (fibroblast) ( 56 ) , L3.6p1 (pancreatic) ( 6 ) , Ls174T (intestinal) ( 50 ) , lung ( 33 ) , MCF-7 (breast cell) ( 7 , 38 ) , MDCK (epithelial) ( 47 , 53 ) , MEF (fibroblast) [axin 2 (mouse), homozygous knockout] ( 34 ) , mesangial ( 29 ) , MKN-45 (gastric) ( 15 ) , MKN-74 (gastric) ( 39 ) , NCI-H520 (squamous) ( 30 ) , oocyte ( 57 ) , Rat2 (fibroblast) ( 57 ) , SKBr3 (breast cell) ( 54 ) , SNU16Ad (gastric) ( 39 ) , SUM159 (breast cell) ( 7 ) , SW480 (intestinal) ( 31 , 35 , 53 ) , SW620 (intestinal) ( 31 ) , U2OS (bone cell) ( 34 )

Upstream Regulation
Regulatory protein:
AurA (human) ( 39 ) , axin 1 (human) ( 56 ) , CDH2 (human) ( 53 ) , CTNNB1 (human) ( 37 ) , DDX5 (human) ( 44 ) , DKK1 (human) ( 29 ) , GSKIP (human) ( 4 ) , Merlin (human) ( 7 ) , PPP2R2A (human) ( 6 ) , SFRP2 (human) ( 32 ) , WNT5A (human) ( 38 )
Putative in vivo kinases:
GSK3B (human) ( 57 ) , IKKA (human) ( 51 ) , JNK1 (human) ( 36 )
Kinases, in vitro:
GSK3A (human) ( 57 ) , GSK3B (human) ( 40 , 46 , 56 ) , JNK1 (human) ( 36 )
Phosphatases, in vitro:
PPP2CA (human) ( 40 )
Treatments:
anti-FGF19 ( 41 ) , calyculin_A ( 31 ) , cAMP_analog ( 46 ) , CK1-7 ( 56 ) , colforsin ( 45 ) , CTGF ( 29 ) , H-89 ( 56 ) , IWR-1 ( 2 ) , KN-92 ( 48 ) , KN-93 ( 48 ) , KY1022 ( 3 ) , lithium ( 31 , 53 , 55 , 56 , 57 , 58 ) , LLnL ( 55 , 58 ) , LY2090314 ( 12 ) , MG132 ( 4 , 41 , 53 ) , okadaic_acid ( 36 ) , SB216763 ( 42 ) , SP600125 ( 36 ) , W-5 ( 48 ) , W-7 ( 48 ) , Wnt ( 55 , 57 , 58 ) , Wnt3a ( 34 )

Downstream Regulation
Effects of modification on CTNNB1:
intracellular localization ( 35 , 39 , 44 ) , molecular association, regulation ( 36 ) , protein degradation ( 7 , 39 , 40 , 43 , 56 ) , ubiquitination ( 47 )
Effects of modification on biological processes:
cell adhesion, altered ( 36 ) , cell growth, altered ( 39 , 54 ) , cytoskeletal reorganization ( 34 ) , transcription, altered ( 39 , 43 ) , transcription, inhibited ( 16 )
Induce interaction with:
CBLL1 (mouse) ( 36 )

Disease / Diagnostics Relevance
Relevant diseases:
colorectal cancer ( 57 )

References 

1

Lee SK, et al. (2018) β-Catenin-RAS interaction serves as a molecular switch for RAS degradation via GSK3β. EMBO Rep 19
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2

Mashima T, et al. (2017) mTOR signaling mediates resistance to tankyrase inhibitors in Wnt-driven colorectal cancer. Oncotarget 8, 47902-47915
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3

Cho YH, et al. (2016) KY1022, a small molecule destabilizing Ras via targeting the Wnt/β-catenin pathway, inhibits development of metastatic colorectal cancer. Oncotarget 7, 81727-81740
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4

Dema A, et al. (2016) The A-Kinase Anchoring Protein (AKAP) Glycogen Synthase Kinase 3β Interaction Protein (GSKIP) Regulates β-Catenin through Its Interactions with Both Protein Kinase A (PKA) and GSK3β. J Biol Chem 291, 19618-30
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5

Li CC, et al. (2016) Enhancement of β-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals. Proc Natl Acad Sci U S A 113, 5946-51
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6

Hein AL, et al. (2016) PR55α Subunit of Protein Phosphatase 2A Supports the Tumorigenic and Metastatic Potential of Pancreatic Cancer Cells by Sustaining Hyperactive Oncogenic Signaling. Cancer Res 76, 2243-53
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7

Morrow KA, et al. (2016) Loss of tumor suppressor Merlin results in aberrant activation of Wnt/β-catenin signaling in cancer. Oncotarget 7, 17991-8005
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8

Panneerselvam J, et al. (2015) Phosphorylation of interleukin (IL)-24 is required for mediating its anti-cancer activity. Oncotarget 6, 16271-86
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9

Iaconelli J, et al. (2015) HDAC6 inhibitors modulate Lys49 acetylation and membrane localization of β-catenin in human iPSC-derived neuronal cells. ACS Chem Biol 10, 883-90
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10

Llado V, et al. (2015) Repression of Intestinal Stem Cell Function and Tumorigenesis through Direct Phosphorylation of β-Catenin and Yap by PKCζ Cell Rep
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11

Xue J, et al. (2015) Tumour suppressor TRIM33 targets nuclear β-catenin degradation. Nat Commun 6, 6156
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12

Atkinson JM, et al. (2015) Activating the Wnt/β-Catenin Pathway for the Treatment of Melanoma--Application of LY2090314, a Novel Selective Inhibitor of Glycogen Synthase Kinase-3. PLoS One 10, e0125028
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13

Vinyoles M, et al. (2014) Multivesicular GSK3 sequestration upon Wnt signaling is controlled by p120-catenin/cadherin interaction with LRP5/6. Mol Cell 53, 444-57
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14

Schofield AV, Gamell C, Bernard O (2013) Tubulin polymerization promoting protein 1 (TPPP1) increases β-catenin expression through inhibition of HDAC6 activity in U2OS osteosarcoma cells. Biochem Biophys Res Commun 436, 571-7
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15

Zhou J (2012) CST Curation Set: 14009; Year: 2012; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb Cat#: 8954, Phospho-Tyrosine Rabbit mAb (p-Tyr-1000) Immunoaffinity Beads Cat#: 8876
Curated Info

16

Imajo M, et al. (2012) A molecular mechanism that links Hippo signalling to the inhibition of Wnt/β-catenin signalling. EMBO J 31, 1109-22
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17

Du C, et al. (2012) Beta-catenin phosphorylated at threonine 120 antagonizes generation of active beta-catenin by spatial localization in trans-Golgi network. PLoS One 7, e33830
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18

Krejci P, et al. (2012) Receptor Tyrosine Kinases Activate Canonical WNT/β-Catenin Signaling via MAP Kinase/LRP6 Pathway and Direct β-Catenin Phosphorylation. PLoS One 7, e35826
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19

Li L, et al. (2011) GLIPR1 suppresses prostate cancer development through targeted oncoprotein destruction. Cancer Res 71, 7694-704
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20

Rikova K (2011) CST Curation Set: 13113; Year: 2011; Biosample/Treatment: cell line, MGH-1/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

21

Rikova K (2011) CST Curation Set: 13114; Year: 2011; Biosample/Treatment: cell line, MGH-2/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

22

Rikova K (2011) CST Curation Set: 13115; Year: 2011; Biosample/Treatment: cell line, MGH-3/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

23

Rikova K (2011) CST Curation Set: 13116; Year: 2011; Biosample/Treatment: cell line, MGH-4/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

24

Rikova K (2011) CST Curation Set: 13117; Year: 2011; Biosample/Treatment: cell line, MGH-5/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

25

Rikova K (2011) CST Curation Set: 13118; Year: 2011; Biosample/Treatment: cell line, MGH-6/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

26

Rikova K (2011) CST Curation Set: 13119; Year: 2011; Biosample/Treatment: cell line, MGH-7/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

27

Rikova K (2011) CST Curation Set: 13121; Year: 2011; Biosample/Treatment: cell line, MGH-9/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

28

Rikova K (2011) CST Curation Set: 13122; Year: 2011; Biosample/Treatment: cell line, MGH-10/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

29

Rooney B, et al. (2011) CTGF/CCN2 activates canonical Wnt signalling in mesangial cells through LRP6: implications for the pathogenesis of diabetic nephropathy. FEBS Lett 585, 531-8
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30

Rikova K (2011) CST Curation Set: 10911; Year: 2011; Biosample/Treatment: cell line, NCI-H520/untreated; Disease: non-small cell squamous cell lung carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

31

Kwon IK, et al. (2010) PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4. Oncogene 29, 3423-34
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32

Yamamura S, et al. (2010) Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer. Mol Cancer Ther 9, 1680-7
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33

Rikova K (2010) CST Curation Set: 9757; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

34

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35

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36

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37

Wu H, Symes K, Seldin DC, Dominguez I (2009) Threonine 393 of beta-catenin regulates interaction with Axin. J Cell Biochem 108, 52-63
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38

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39

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40

Su Y, et al. (2008) APC is essential for targeting phosphorylated beta-catenin to the SCFbeta-TrCP ubiquitin ligase. Mol Cell 32, 652-61
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41

Pai R, et al. (2008) Inhibition of fibroblast growth factor 19 reduces tumor growth by modulating beta-catenin signaling. Cancer Res 68, 5086-95
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42

Coluccia AM, et al. (2007) Bcr-Abl stabilizes beta-catenin in chronic myeloid leukemia through its tyrosine phosphorylation. EMBO J 26, 1456-66
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43

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44

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45

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46

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47

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48

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49

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50

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51

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52

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53

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54

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55

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56

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57

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58

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59

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60

Orford K, et al. (1997) Serine phosphorylation-regulated ubiquitination and degradation of beta-catenin. J Biol Chem 272, 24735-8
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