Ser32
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Home > Phosphorylation Site Page: > Ser32  -  PFKFB2 (human)

Site Information
EKKCSWAsYMTNSPT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 23003811

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 1 , 3 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 3 )
Relevant cell line - cell type - tissue:
COS (fibroblast) ( 1 , 3 ) , hepatocyte ( 1 , 3 )

Upstream Regulation
Treatments:
glucagon ( 1 , 3 ) , glucose ( 3 ) , H-89 ( 1 ) , xylitol ( 3 )

Downstream Regulation
Effects of modification on PFKFB2:
enzymatic activity, inhibited ( 2 ) , intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
transcription, altered ( 3 )
Induce interaction with:
FBPase 2 (human) ( 1 )

References 

1

Cullen KS, et al. (2014) Glucagon induces translocation of glucokinase from the cytoplasm to the nucleus of hepatocytes by transfer between 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2 and the glucokinase regulatory protein. Biochim Biophys Acta 1843, 1123-34
24566088   Curated Info

2

Dasgupta T, et al. (2014) A fundamental trade-off in covalent switching and its circumvention by enzyme bifunctionality in glucose homeostasis. J Biol Chem 289, 13010-25
24634222   Curated Info

3

Arden C, et al. (2012) Fructose 2,6-bisphosphate is essential for glucose-regulated gene transcription of glucose-6-phosphatase and other ChREBP target genes in hepatocytes. Biochem J 443, 111-23
22214556   Curated Info