Ser358
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Home > Phosphorylation Site Page: > Ser358  -  MLKL (human)

Site Information
ELRKTQTsMSLGTTR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 22996309

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 8 ) , immunoassay ( 2 , 4 , 7 ) , immunoprecipitation ( 6 , 8 ) , mass spectrometry ( 8 ) , mutation of modification site ( 7 , 8 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 ) , western blotting ( 2 , 3 , 4 , 6 , 7 )
Disease tissue studied:
Alzheimer's disease ( 5 ) , breast cancer ( 2 ) , breast adenocarcinoma ( 2 ) , breast cancer, triple negative ( 2 ) , colorectal cancer ( 3 , 4 , 6 , 7 , 8 ) , colorectal carcinoma ( 3 , 4 , 6 , 7 , 8 ) , lymphoma ( 3 , 7 ) , fibrosarcoma of soft tissue ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
RIPK3 (human) ( 4 , 7 , 8 )
Kinases, in vitro:
RIPK3 (human) ( 8 )
Treatments:
adriamycin ( 2 ) , BV6 ( 2 ) , LPS ( 7 ) , Necrostatin-1 ( 7 ) , phenhydan ( 3 ) , resibufogenin ( 4 ) , siRNA ( 4 ) , TNF ( 2 , 3 , 7 ) , virus infection ( 2 ) , Z-VAD-FMK ( 2 , 3 , 7 )

Downstream Regulation
Effects of modification on MLKL:
intracellular localization ( 2 , 5 , 6 , 7 ) , molecular association, regulation ( 7 ) , protein conformation ( 5 , 6 )
Effects of modification on biological processes:
apoptosis, induced ( 2 , 7 ) , carcinogenesis, inhibited ( 4 ) , cell motility, inhibited ( 4 ) , signaling pathway regulation ( 8 ) , transcription, induced ( 4 )
Induce interaction with:
MLKL (human) ( 7 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 5 ) , colorectal cancer ( 4 )

References 

1

Zu R, et al. (2021) Quantitative analysis of phosphoproteome in necroptosis reveals a role of TRIM28 phosphorylation in promoting necroptosis-induced cytokine production. Cell Death Dis 12, 994
34689152   Curated Info

2

Zhang J, et al. (2021) Necroptotic virotherapy of oncolytic alphavirus M1 cooperated with Doxorubicin displays promising therapeutic efficacy in TNBC. Oncogene
34155344   Curated Info

3

Moerke C, et al. (2018) The anticonvulsive Phenhydan suppresses extrinsic cell death. Cell Death Differ
30442947   Curated Info

4

Han Q, et al. (2018) Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis. J Transl Med 16, 201
30029665   Curated Info

5

Caccamo A, et al. (2017) Necroptosis activation in Alzheimer's disease. Nat Neurosci 20, 1236-1246
28758999   Curated Info

6

Yoon S, Bogdanov K, Kovalenko A, Wallach D (2016) Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it. Cell Death Differ 23, 253-60
26184911   Curated Info

7

Wang H, et al. (2014) Mixed Lineage Kinase Domain-like Protein MLKL Causes Necrotic Membrane Disruption upon Phosphorylation by RIP3. Mol Cell 54, 133-46
24703947   Curated Info

8

Sun L, et al. (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148, 213-27
22265413   Curated Info