Ser184
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Home > Phosphorylation Site Page: > Ser184  -  14-3-3 zeta (human)

Site Information
FyYEILNsPEKACSL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449855

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 5 ) , mass spectrometry ( 3 , 4 , 6 ) , microscopy-colocalization with upstream kinase ( 7 ) , mutation of modification site ( 2 , 5 , 7 ) , phospho-antibody ( 1 , 8 ) , western blotting ( 1 , 8 )
Disease tissue studied:
Alzheimer's disease ( 6 ) , bone cancer ( 4 ) , osteosarcoma ( 4 ) , colorectal cancer ( 8 ) , colorectal carcinoma ( 8 ) , FTLD ( 3 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 1 , 3 , 6 ) , COS (fibroblast) ( 7 ) , E.coli (bacterial) ( 2 , 5 ) , HCT116 (intestinal) ( 8 ) , leukocyte-blood ( 4 )

Upstream Regulation
Regulatory protein:
JNK1 (human) ( 7 ) , SYN1 (human) ( 1 )
Putative in vivo kinases:
JNK1 (human) ( 7 )
Kinases, in vitro:
JNK1 (human) ( 7 , 8 )
Treatments:
anisomycin ( 8 ) , doxycycline ( 1 ) , rotenone ( 1 ) , SP600125 ( 8 ) , UV ( 7 )

Downstream Regulation
Effects of modification on 14-3-3 zeta:
molecular association, regulation ( 2 , 7 , 8 ) , phosphorylation ( 7 ) , protein conformation ( 5 ) , protein stabilization ( 5 )
Induce interaction with:
HSP20 (human) ( 2 )
Inhibit interaction with:
BAX (human) ( 8 )

References 

1

Slone SR, et al. (2015) Increased 14-3-3 phosphorylation observed in Parkinson's disease reduces neuroprotective potential of 14-3-3 proteins. Neurobiol Dis 79, 1-13
25862939   Curated Info

2

Sluchanko NN, et al. (2011) Phosphomimicking mutations of human 14-3-3ΞΆ affect its interaction with tau protein and small heat shock protein HspB6. Arch Biochem Biophys 506, 24-34
21081103   Curated Info

3

Herskowitz JH, et al. (2010) Phosphoproteomic Analysis Reveals Site-Specific Changes in GFAP and NDRG2 Phosphorylation in Frontotemporal Lobar Degeneration. J Proteome Res 9, 6368-79
20886841   Curated Info

4

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
20058876   Curated Info

5

Sluchanko NN, et al. (2008) Effect of mutations mimicking phosphorylation on the structure and properties of human 14-3-3zeta. Arch Biochem Biophys 477, 305-12
18559254   Curated Info

6

Xia Q, et al. (2008) Phosphoproteomic analysis of human brain by calcium phosphate precipitation and mass spectrometry. J Proteome Res 7, 2845-51
18510355   Curated Info

7

Sunayama J, Tsuruta F, Masuyama N, Gotoh Y (2005) JNK antagonizes Akt-mediated survival signals by phosphorylating 14-3-3. J Cell Biol 170, 295-304
16009721   Curated Info

8

Tsuruta F, et al. (2004) JNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins. EMBO J 23, 1889-99
15071501   Curated Info

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