Tyr658
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Home > Phosphorylation Site Page: > Tyr658  -  CDH5 (human)

Site Information
GEMDTTSyDVSVLNs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456048

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 6 ) , mutation of modification site ( 1 , 2 , 3 , 5 , 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Disease tissue studied:
leukemia ( 3 ) , melanoma skin cancer ( 2 , 3 )
Relevant cell line - cell type - tissue:
1205Lu (melanocyte) ( 2 , 3 ) , A375 (melanocyte) ( 2 ) , BAEC (endothelial) ( 1 ) , COS7 (fibroblast) ( 3 ) , endothelial ( 1 , 5 ) , HL60 (myeloid) ( 3 ) , HUVEC (endothelial) ( 1 , 3 , 4 ) , UACC-903 ( 2 ) , WM35 (melanocyte) ( 2 , 3 )

Upstream Regulation
Regulatory protein:
BRAF (human) ( 2 ) , CD44 (human) ( 3 ) , F3 (human) ( 2 ) , RRas (human) ( 4 )
Putative in vivo kinases:
Src (human) ( 6 )
Treatments:
chondroitinase AC ( 3 ) , culturing_of_cells ( 3 ) , hirudin ( 2 ) , neuraminidase ( 3 ) , platlet-free_plasma ( 2 ) , vanadate ( 6 ) , VEGF ( 4 )

Downstream Regulation
Effects of modification on CDH5:
molecular association, regulation ( 1 , 5 , 6 )
Effects of modification on biological processes:
cell adhesion, altered ( 5 ) , cell motility, altered ( 5 ) , cell motility, induced ( 3 ) , signaling pathway regulation ( 5 )
Induce interaction with:
GPSM2 (mouse) ( 1 )
Inhibit interaction with:
CTNND1 (human) ( 5 , 6 ) , CTNND1 (mouse) ( 1 )

References 

1

Conway DE, et al. (2017) VE-Cadherin Phosphorylation Regulates Endothelial Fluid Shear Stress Responses through the Polarity Protein LGN. Curr Biol 27, 2219-2225.e5
28712573   Curated Info

2

Zhang P, et al. (2016) Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown. J Biol Chem 291, 2087-106
26504080   Curated Info

3

Zhang P, et al. (2014) CD44 variant, but not standard CD44 isoforms, mediate disassembly of endothelial VE-cadherin junction on metastatic melanoma cells. FEBS Lett 588, 4573-82
25447529   Curated Info

4

Sawada J, et al. (2012) Small GTPase R-Ras Regulates Integrity and Functionality of Tumor Blood Vessels. Cancer Cell 22, 235-49
22897853   Curated Info

5

Hatanaka K, Simons M, Murakami M (2011) Phosphorylation of VE-cadherin controls endothelial phenotypes via p120-catenin coupling and Rac1 activation. Am J Physiol Heart Circ Physiol 300, H162-72
21037229   Curated Info

6

Potter MD, Barbero S, Cheresh DA (2005) Tyrosine phosphorylation of VE-cadherin prevents binding of p120- and beta-catenin and maintains the cellular mesenchymal state. J Biol Chem 280, 31906-12
16027153   Curated Info