Ser191
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Home > Phosphorylation Site Page: > Ser191  -  GPIbB (human)

Site Information
ARAAARLsLtDPLVA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456005

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 16 ) , [32P] bio-synthetic labeling ( 16 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 9 , 10 , 11 , 12 , 13 ) , mutation of modification site ( 8 , 14 , 15 ) , phospho-antibody ( 2 , 14 , 15 ) , phosphoamino acid analysis ( 16 ) , phosphopeptide mapping ( 16 ) , western blotting ( 2 , 15 , 16 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 15 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 15 ) , hepatocellular carcinoma, surrounding tissue ( 9 ) , ovarian cancer ( 3 ) , pancreatic ductal adenocarcinoma ( 5 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 7 ) , 'pancreatic, ductal'-pancreas ( 5 ) , breast ( 1 ) , CHO (fibroblast) ( 8 ) , HEL (erythroid) ( 15 ) , hepatocyte-liver ( 9 ) , K562 (erythroid) ( 6 , 11 ) , leukocyte-blood ( 10 ) , liver ( 4 ) , ovary ( 3 ) , platelet-blood ( 13 , 15 , 16 ) , T lymphocyte-blood ( 12 )

Upstream Regulation
Kinases, in vitro:
PKACA (cow) ( 16 )
Treatments:
alprostadil ( 16 ) , colforsin ( 14 ) , mPKI ( 14 )

Downstream Regulation
Effects of modification on GPIbB:
molecular association, regulation ( 15 ) , receptor desensitization, altered ( 14 )
Effects of modification on biological processes:
cell adhesion, altered ( 14 )
Induce interaction with:
14-3-3 zeta (human) ( 15 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Manchev VT, et al. (2014) A new form of macrothrombocytopenia induced by a germ-line mutation in the PRKACG gene. Blood 124, 2554-63
25061177   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

5

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

8

David T, et al. (2010) The platelet glycoprotein GPIbbeta intracellular domain participates in von Willebrand factor induced-filopodia formation independently of the Ser 166 phosphorylation site. J Thromb Haemost 8, 1077-87
19694944   Curated Info

9

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

10

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
20058876   Curated Info

11

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

12

Carrascal M, et al. (2008) Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment. J Proteome Res 7, 5167-76
19367720   Curated Info

13

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

14

Bodnar RJ, et al. (2002) Regulation of glycoprotein Ib-IX-von Willebrand factor interaction by cAMP-dependent protein kinase-mediated phosphorylation at Ser 166 of glycoprotein Ib(beta). J Biol Chem 277, 47080-7
12361948   Curated Info

15

Calverley DC, Kavanagh TJ, Roth GJ (1998) Human signaling protein 14-3-3zeta interacts with platelet glycoprotein Ib subunits Ibalpha and Ibbeta. Blood 91, 1295-303
9454760   Curated Info

16

Wardell MR, et al. (1989) Platelet glycoprotein Ib beta is phosphorylated on serine 166 by cyclic AMP-dependent protein kinase. J Biol Chem 264, 15656-61
2504723   Curated Info