Ser242
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.5
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser242  -  pyrin (human)

Site Information
SGKMRPRsLEVTIST   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455899

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , immunoprecipitation ( 5 , 7 ) , mass spectrometry ( 6 , 7 , 8 ) , mutation of modification site ( 2 , 4 , 5 , 9 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 7 )
Disease tissue studied:
HER2 positive breast cancer ( 6 ) , luminal A breast cancer ( 6 ) , luminal B breast cancer ( 6 ) , breast cancer, triple negative ( 6 ) , leukemia ( 1 , 2 ) , acute myelogenous leukemia ( 1 , 2 , 8 ) , lymphoma ( 2 , 4 )
Relevant cell line - cell type - tissue:
blood ( 8 ) , breast ( 6 ) , HEK293T (epithelial) ( 7 ) , HeLa (cervical) ( 9 ) , macrophage-bone marrow ( 5 ) , monocyte ( 3 , 4 ) , mononuclear-blood ( 5 ) , THP1 (myeloid) ( 1 , 2 ) , U-937 (myeloid) ( 2 , 4 )

Upstream Regulation
Regulatory protein:
CDC42 (human) ( 2 )
Putative in vivo kinases:
PKN1 (human) ( 5 )
Kinases, in vitro:
PKN1 (human) ( 5 ) , PKN2 (human) ( 5 )
Treatments:
bacterial infection ( 1 ) , C. difficile Cytotoxin TcdA ( 4 ) , calyculin_A ( 1 ) , Clostridioides difficile toxin B ( 2 , 3 ) , etiocholanolone ( 4 ) , IPA-3 ( 2 ) , LPS ( 3 ) , pregnanolone ( 4 ) , tautomycetin ( 1 ) , UCN-01 ( 4 )

Downstream Regulation
Effects of modification on pyrin:
activity, inhibited ( 2 , 3 , 7 ) , intracellular localization ( 9 ) , molecular association, regulation ( 2 , 5 , 7 , 9 )
Induce interaction with:
14-3-3 beta (human) ( 7 ) , 14-3-3 beta (mouse) ( 2 ) , 14-3-3 epsilon (human) ( 5 ) , 14-3-3 theta (human) ( 9 )

Disease / Diagnostics Relevance
Relevant diseases:
PAAND ( 7 )

References 

1

Malik HS, et al. (2023) Phosphoprotein phosphatase activity positively regulates oligomeric pyrin to trigger inflammasome assembly in phagocytes. mBio, e0206623
37787552   Curated Info

2

Spel L, et al. (2022) CDC42 regulates PYRIN inflammasome assembly. Cell Rep 41, 111636
36384121   Curated Info

3

Mangan MSJ, et al. (2022) Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever. PLoS Biol 20, e3001351
36342970   Curated Info

4

Magnotti F, et al. (2022) Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism. Cell Rep 41, 111472
36223753   Curated Info

5

Park YH, Wood G, Kastner DL, Chae JJ (2016) Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol 17, 914-21
27270401   Curated Info

6

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

7

Masters SL, et al. (2016) Familial autoinflammation with neutrophilic dermatosis reveals a regulatory mechanism of pyrin activation. Sci Transl Med 8, 332ra45
27030597   Curated Info

8

Cobbold M, et al. (2013) MHC class I-associated phosphopeptides are the targets of memory-like immunity in leukemia. Sci Transl Med 5, 203ra125
24048523   Curated Info

9

Jéru I, et al. (2005) Interaction of pyrin with 14.3.3 in an isoform-specific and phosphorylation-dependent manner regulates its translocation to the nucleus. Arthritis Rheum 52, 1848-57
15934090   Curated Info