Tyr240
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Home > Phosphorylation Site Page: > Tyr240  -  SHC1 iso2 (mouse)

Site Information
EPPDHQyyNDFPGKE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448510

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 3 , 6 ) , mutation of modification site ( 1 , 2 , 5 ) , phospho-antibody ( 4 ) , western blotting ( 1 , 2 , 4 )
Disease tissue studied:
breast cancer ( 5 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 6 ) , L3.6p1 (pancreatic) ( 1 ) , mammary gland ( 5 ) , MEF (fibroblast) ( 3 , 4 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 3 ) , NMuMG (epithelial) ( 2 )

Upstream Regulation
Regulatory protein:
PAG (rat) ( 4 )
Treatments:
EGF ( 3 ) , PDGF ( 3 )

Downstream Regulation
Effects of modification on SHC1 iso2:
molecular association, regulation ( 5 ) , phosphorylation ( 5 )
Effects of modification on biological processes:
cell motility, altered ( 5 ) , cell motility, induced ( 2 , 5 )
Induce interaction with:
GRB2 (mouse) ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
lung cancer ( 2 )

References 

1

Huang H, et al. (2016) Up-regulation of N-cadherin by Collagen I-activated Discoidin Domain Receptor 1 in Pancreatic Cancer Requires the Adaptor Molecule Shc1. J Biol Chem 291, 23208-23223
27605668   Curated Info

2

Northey JJ, et al. (2013) Distinct Phosphotyrosine-dependent Functions of the ShcA Adaptor Protein Are Required for Transforming Growth Factor β (TGFβ)-induced Breast Cancer Cell Migration, Invasion, and Metastasis. J Biol Chem 288, 5210-22
23277357   Curated Info

3

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

4

Oneyama C, et al. (2008) The lipid raft-anchored adaptor protein Cbp controls the oncogenic potential of c-Src. Mol Cell 30, 426-36
18498747   Curated Info

5

Ursini-Siegel J, et al. (2008) ShcA signalling is essential for tumour progression in mouse models of human breast cancer. EMBO J 27, 910-20
18273058   Curated Info

6

Moritz A (2003) CST Curation Set: 208; Year: 2003; Biosample/Treatment: cell line, 3T3/NRG1; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info