Lys526

Site Information
LIHMGLLkSEDKIKA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2251700

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 4 ) , mass spectrometry (in vitro) ( 2 ) , modification-specific antibody ( 4 ) , mutation of modification site ( 4 ) , peptide sequencing ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:	COS7 (fibroblast) ( 4 ) , E.coli (bacterial) ( 4 ) , liver ( 4 )

Downstream Regulation
Effects of modification on RANGAP1:	intracellular localization ( 4 ) , sumoylation ( 3 )

References
1	Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171 29079793 Curated Info
2	Pedrioli PG, et al. (2006) Automated identification of SUMOylation sites using mass spectrometry and SUMmOn pattern recognition software. Nat Methods 3, 533-9 16791211 Curated Info
3	Reverter D, Lima CD (2005) Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex. Nature 435, 687-92 15931224 Curated Info
4	Mahajan R, Gerace L, Melchior F (1998) Molecular characterization of the SUMO-1 modification of RanGAP1 and its role in nuclear envelope association. J Cell Biol 140, 259-70 9442102 Curated Info