Ser264

Site Information
VELHsPQsLPRGskA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2238600

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 4 ) , mutation of modification site ( 1 , 5 , 6 , 7 ) , phospho-antibody ( 3 , 6 , 7 ) , western blotting ( 3 , 5 , 6 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 1 ) , kidney ( 2 , 7 ) , MDCK (epithelial) ( 5 , 7 ) , mpkCCD (renal) ( 3 , 4 ) , oocyte ( 6 )

Downstream Regulation
Effects of modification on AQP2:	intracellular localization ( 5 ) , molecular association, regulation ( 1 )
Induce interaction with:	14-3-3 theta (human) ( 1 )

References
1	Moeller HB, et al. (2016) Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ. J Biol Chem 291, 2469-84 26645691 Curated Info
2	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
3	Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23 20089674 Curated Info
4	Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7 20139300 Curated Info
5	Moeller HB, Praetorius J, Rützler MR, Fenton RA (2010) Phosphorylation of aquaporin-2 regulates its endocytosis and protein-protein interactions. Proc Natl Acad Sci U S A 107, 424-9 19966308 Curated Info
6	Moeller HB, Macaulay N, Knepper MA, Fenton RA (2009) Role of multiple phosphorylation sites in the COOH-terminal tail of aquaporin-2 for water transport: evidence against channel gating. Am J Physiol Renal Physiol 296, F649-57 19144687 Curated Info
7	Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27 18606813 Curated Info