Ser264
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Home > Phosphorylation Site Page: > Ser264  -  AQP2 (rat)

Site Information
VELHsPQsLPRGskA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2238600

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 9 , 12 , 15 , 16 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 11 , 12 , 13 , 14 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 7 , 11 , 13 )
Disease tissue studied:
kidney cancer ( 14 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 2 , 3 , 4 , 7 , 11 , 12 , 13 , 15 , 16 ) , kidney ( 6 , 9 , 14 ) , LLC-PK1 (renal) ( 1 ) , MDCK (epithelial) ( 6 ) , mpkCCD (renal) ( 11 )

Upstream Regulation
Treatments:
butaprost ( 10 ) , calyculin_A ( 3 , 4 ) , cAMP_analog ( 12 ) , CAY10580 ( 10 ) , desmopressin ( 2 , 5 , 12 , 13 , 14 , 15 , 16 ) , FK506 ( 3 , 4 ) , H-89 ( 12 ) , IBMX ( 2 ) , ibuprofen ( 7 ) , meloxicam ( 7 ) , Mn(2+) ( 1 ) , PGE2 ( 10 ) , roflumilast N-oxide ( 2 ) , tolvaptan ( 8 ) , vasopressin ( 1 , 6 , 8 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 6 , 14 ) , ubiquitination ( 3 )
Effects of modification on biological processes:
exocytosis, induced ( 3 )

References 

1

Lei L, et al. (2017) Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. Am J Physiol Renal Physiol, ajprenal003912017
29046300   Curated Info

2

Umejiego EN, Wang Y, Knepper MA, Chou CL (2017) Roflumilast and aquaporin-2 regulation in rat renal inner medullary collecting duct. Physiol Rep 5
28108651   Curated Info

3

Ren H, et al. (2016) Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane. Am J Physiol Renal Physiol 311, F1189-F1197
27488997   Curated Info

4

Al-Bataineh MM, et al. (2016) Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells. Am J Physiol Renal Physiol 311, F890-F900
27534994   Curated Info

5

Choi HJ, Jung HJ, Kwon TH (2015) Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308, F737-48
25651562   Curated Info

6

Rice WL, et al. (2015) Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PLoS One 10, e0131719
26147297   Curated Info

7

Ren H, et al. (2015) NSAIDs Alter Phosphorylated Forms of AQP2 in the Inner Medullary Tip. PLoS One 10, e0141714
26517129   Curated Info

8

Miranda CA, Lee JW, Chou CL, Knepper MA (2014) Tolvaptan as a tool in renal physiology. Am J Physiol Renal Physiol 306, F359-66
24305472   Curated Info

9

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

10

Olesen ET, et al. (2011) Vasopressin-independent targeting of aquaporin-2 by selective E-prostanoid receptor agonists alleviates nephrogenic diabetes insipidus. Proc Natl Acad Sci U S A 108, 12949-54
21768374   Curated Info

11

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

12

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info

13

Yu MJ, et al. (2008) Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct. Am J Physiol Cell Physiol 295, C661-78
18596208   Curated Info

14

Fenton RA, et al. (2008) Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin. Proc Natl Acad Sci U S A 105, 3134-9
18287043   Curated Info

15

Hoffert JD, et al. (2007) An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins. J Proteome Res 6, 3501-8
17683130   Curated Info

16

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info