Ser660
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Home > Phosphorylation Site Page: > Ser660  -  TBC1D1 (mouse)

Site Information
PRLNPSAssPNFFKY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2228809

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , mass spectrometry ( 2 , 3 , 5 , 6 , 7 , 8 , 9 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 , 4 )
Disease tissue studied:
pancreatic cancer ( 1 ) , pancreatic carcinoma ( 1 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'muscle, skeletal' ( 4 , 9 ) , brain ( 5 , 7 ) , C2C12 (myoblast) ( 8 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 3 ) , kidney ( 6 ) , PANC-1 (pancreatic) ( 1 ) , pancreas ( 1 ) , SW1990 (pancreatic) ( 1 ) , testis ( 6 )

Upstream Regulation
Regulatory protein:
MICAL1 (human) ( 1 )
Putative in vivo kinases:
AMPKA1 (mouse) ( 9 )
Kinases, in vitro:
Akt1 (human) ( 9 ) , AMPKA1 (human) ( 9 )
Treatments:
acadesine ( 9 ) , cyclochalasin D ( 1 ) , exercise ( 4 ) , insulin ( 4 , 9 ) , muscle contraction ( 4 , 9 )

Downstream Regulation
Effects of modification on TBC1D1:
molecular association, regulation ( 1 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell growth, induced ( 1 ) , cell motility, induced ( 1 ) , signaling pathway regulation ( 1 ) , transcription, induced ( 1 )
Induce interaction with:
FZD7 (human) ( 1 )

References 

1

Cai K, et al. (2022) MICAL1 facilitates pancreatic cancer proliferation, migration, and invasion by activating WNT/β-catenin pathway. J Transl Med 20, 528
36371204   Curated Info

2

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

3

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

4

Treebak JT, et al. (2014) Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol 592, 351-75
24247980   Curated Info

5

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

6

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

7

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

8

Peck GR, et al. (2009) Insulin-stimulated phosphorylation of the Rab GTPase-activating protein TBC1D1 regulates GLUT4 translocation. J Biol Chem 284, 30016-23
19740738   Curated Info

9

Taylor EB, et al. (2008) Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J Biol Chem 283, 9787-96
18276596   Curated Info

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