Thr499
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Home > Phosphorylation Site Page: > Thr499  -  TBC1D1 (mouse)

Site Information
NRAKRsLtEsLEsIL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2228808

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 4 )
Relevant cell line - cell type - tissue:
'fat, brown' ( 3 ) , 'muscle, skeletal' ( 7 ) , 3T3-L1 (fibroblast) ( 4 ) , brain ( 3 ) , C2C12 (myoblast) ( 4 , 5 , 6 ) , heart ( 3 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 1 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 1 ) , HL-1 (myocyte) ( 1 ) , kidney ( 3 ) , liver ( 3 ) , lung ( 3 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (mouse) ( 7 )
Kinases, in vitro:
Akt1 (human) ( 7 ) , AMPKA1 (human) ( 7 )
Treatments:
acadesine ( 7 ) , insulin ( 4 )

Downstream Regulation
Effects of modification on TBC1D1:
activity, inhibited ( 4 )

References 

1

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

4

Peck GR, et al. (2009) Insulin-stimulated phosphorylation of the Rab GTPase-activating protein TBC1D1 regulates GLUT4 translocation. J Biol Chem 284, 30016-23
19740738   Curated Info

5

Guo A (2009) CST Curation Set: 6167; Year: 2009; Biosample/Treatment: cell line, C2C12/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Guo A (2009) CST Curation Set: 6164; Year: 2009; Biosample/Treatment: cell line, C2C12/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

7

Taylor EB, et al. (2008) Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J Biol Chem 283, 9787-96
18276596   Curated Info

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