Ser700

Site Information
KKLHssssVPNFLKF SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2228807

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 5 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'fat, brown' ( 5 ) , 'muscle, skeletal' ( 4 , 9 ) , brain ( 5 , 6 ) , C2C12 (myoblast) ( 7 ) , heart ( 5 ) , Hepa 1-6 (epithelial) ( 8 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 3 ) , kidney ( 5 ) , lung ( 5 ) , pancreas ( 5 ) , spleen ( 5 ) , testis ( 5 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 2 , 3 , 5 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 4 ) , western blotting ( 4 )

Relevant cell line - cell type - tissue:

'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'fat, brown' ( 5 ) , 'muscle, skeletal' ( 4 , 9 ) , brain ( 5 , 6 ) , C2C12 (myoblast) ( 7 ) , heart ( 5 ) , Hepa 1-6 (epithelial) ( 8 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 3 ) , kidney ( 5 ) , lung ( 5 ) , pancreas ( 5 ) , spleen ( 5 ) , testis ( 5 )

Upstream Regulation
Putative in vivo kinases:	AMPKA1 (mouse) ( 9 )
Kinases, in vitro:	Akt1 (human) ( 9 ) , AMPKA1 (human) ( 9 )
Treatments:	acadesine ( 9 ) , exercise ( 4 ) , insulin ( 4 , 9 ) , muscle contraction ( 4 , 9 )

References
1	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
2	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
3	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
4	Treebak JT, et al. (2014) Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol 592, 351-75 24247980 Curated Info
5	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
6	Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9 20415495 Curated Info
7	Peck GR, et al. (2009) Insulin-stimulated phosphorylation of the Rab GTPase-activating protein TBC1D1 regulates GLUT4 translocation. J Biol Chem 284, 30016-23 19740738 Curated Info
8	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info
9	Taylor EB, et al. (2008) Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J Biol Chem 283, 9787-96 18276596 Curated Info