Ser262
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Home > Phosphorylation Site Page: > Ser262  -  FOXO4 (human)

Site Information
TFRPRSssNAsSVsT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448517

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 , 4 , 5 , 6 ) , phospho-antibody ( 1 , 4 , 5 ) , phosphoamino acid analysis ( 6 ) , phosphopeptide mapping ( 6 ) , western blotting ( 1 , 4 , 5 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Akt1 (human) ( 1 , 4 , 5 , 6 )
Kinases, in vitro:
Akt1 (human) ( 1 , 4 , 6 )
Treatments:
EGF ( 6 ) , insulin ( 5 , 6 ) , leptomycin_B ( 5 ) , LY294002 ( 6 ) , PDGF ( 6 ) , wortmannin ( 4 , 6 )

Downstream Regulation
Effects of modification on FOXO4:
intracellular localization ( 5 )
Effects of modification on biological processes:
transcription, inhibited ( 5 , 6 )

References 

1

Zhang F, Virshup DM, Cheong JK (2018) Oncogenic RAS-induced CK1α drives nuclear FOXO proteolysis. Oncogene 37, 363-376
28945225   Curated Info

2

Possemato A (2010) CST Curation Set: 10783; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

3

Moritz A (2008) CST Curation Set: 5658; Year: 2008; Biosample/Treatment: cell line, NCI-H3255/wortmannin; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

4

Matsuzaki H, et al. (2005) Regulation of intracellular localization and transcriptional activity of FOXO4 by protein kinase B through phosphorylation at the motif sites conserved among the FOXO family. J Biochem (Tokyo) 138, 485-91
16272144   Curated Info

5

Brownawell AM, Kops GJ, Macara IG, Burgering BM (2001) Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX. Mol Cell Biol 21, 3534-46
11313479   Curated Info

6

Kops GJ, et al. (1999) Direct control of the Forkhead transcription factor AFX by protein kinase B. Nature 398, 630-4
10217147   Curated Info