Ser197
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Home > Phosphorylation Site Page: > Ser197  -  FOXO4 (human)

Site Information
APRRRAAsMDsSSKL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448516

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 17 , 19 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 7 , 8 , 9 , 10 , 12 ) , mutation of modification site ( 15 , 17 , 18 , 19 ) , phospho-antibody ( 6 , 11 , 14 , 15 , 16 , 17 , 18 ) , phosphoamino acid analysis ( 19 ) , phosphopeptide mapping ( 19 ) , western blotting ( 6 , 11 , 14 , 15 , 17 , 18 )
Disease tissue studied:
breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , colorectal cancer ( 14 ) , colorectal carcinoma ( 14 ) , liver cancer ( 6 ) , lung cancer ( 14 ) , non-small cell lung cancer ( 14 ) , non-small cell squamous cell lung carcinoma ( 14 ) , neuroblastoma ( 2 , 12 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
DNAPK (human) ( 13 ) , LKB1 (human) ( 14 )
Putative in vivo kinases:
Akt1 (human) ( 15 , 18 , 19 )
Kinases, in vitro:
Akt1 (human) ( 15 , 19 )
Treatments:
adriamycin ( 13 ) , Akt-I-1 ( 11 ) , EGF ( 19 ) , HGF ( 6 ) , insulin ( 17 , 18 , 19 ) , IQA ( 16 ) , leptomycin_B ( 18 ) , LY294002 ( 11 , 16 , 19 ) , PDGF ( 19 ) , siRNA ( 14 ) , TBB ( 16 ) , wortmannin ( 15 , 19 )

Downstream Regulation
Effects of modification on FOXO4:
intracellular localization ( 15 , 18 )
Effects of modification on biological processes:
apoptosis, altered ( 14 ) , transcription, inhibited ( 15 , 18 , 19 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

5

Rikova K (2012) CST Curation Set: 14275; Year: 2012; Biosample/Treatment: cell line, Tumor pilot study 2mg/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Fafalios A, et al. (2011) A hepatocyte growth factor receptor (Met)-insulin receptor hybrid governs hepatic glucose metabolism. Nat Med 17, 1577-84
22081023   Curated Info

7

Possemato A (2010) CST Curation Set: 10733; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (81E12B5) Rabbit mAb Cat#: 10003
Curated Info

8

Possemato A (2010) CST Curation Set: 10728; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (81E12B5) Rabbit mAb Cat#: 10003
Curated Info

9

Possemato A (2010) CST Curation Set: 10729; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

10

Possemato A (2010) CST Curation Set: 10732; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

11

Singh A, et al. (2010) Protein phosphatase 2A reactivates FOXO3a through a dynamic interplay with 14-3-3 and AKT. Mol Biol Cell 21, 1140-52
20110348   Curated Info

12

Rikova K (2010) CST Curation Set: 9208; Year: 2010; Biosample/Treatment: cell line, LAN-6/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

13

Park J, et al. (2009) DNA-dependent protein kinase-mediated phosphorylation of protein kinase B requires a specific recognition sequence in the C-terminal hydrophobic motif. J Biol Chem 284, 6169-74
19144640   Curated Info

14

Zhong D, et al. (2008) LKB1 is necessary for Akt-mediated phosphorylation of proapoptotic proteins. Cancer Res 68, 7270-7
18794113   Curated Info

15

Matsuzaki H, et al. (2005) Regulation of intracellular localization and transcriptional activity of FOXO4 by protein kinase B through phosphorylation at the motif sites conserved among the FOXO family. J Biochem (Tokyo) 138, 485-91
16272144   Curated Info

16

Di Maira G, et al. (2005) Protein kinase CK2 phosphorylates and upregulates Akt/PKB. Cell Death Differ 12, 668-77
15818404   Curated Info

17

Essers MA, et al. (2004) FOXO transcription factor activation by oxidative stress mediated by the small GTPase Ral and JNK. EMBO J 23, 4802-12
15538382   Curated Info

18

Brownawell AM, Kops GJ, Macara IG, Burgering BM (2001) Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX. Mol Cell Biol 21, 3534-46
11313479   Curated Info

19

Kops GJ, et al. (1999) Direct control of the Forkhead transcription factor AFX by protein kinase B. Nature 398, 630-4
10217147   Curated Info