Ser10

Site Information
tkQTARkstGGkAPr SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 455676

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 47 ) , immunoassay ( 8 , 11 ) , immunoprecipitation ( 1 , 4 , 10 , 13 ) , mass spectrometry ( 1 , 15 , 39 ) , microscopy-colocalization with upstream kinase ( 4 ) , modification-specific antibody ( 10 , 39 ) , mutation of modification site ( 1 , 20 , 21 , 26 ) , phospho-antibody ( 1 , 4 , 5 , 6 , 8 , 9 , 11 , 13 , 17 , 19 , 21 , 22 , 23 , 25 , 26 , 27 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 50 ) , western blotting ( 1 , 6 , 8 , 9 , 13 , 17 , 19 , 21 , 22 , 23 , 25 , 26 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 40 , 44 , 45 , 46 , 47 , 48 , 50 )
Disease tissue studied:	ataxia-telangiectasia ( 44 ) , bone cancer ( 21 ) , breast cancer ( 36 , 40 ) , colorectal cancer ( 45 ) , colorectal carcinoma ( 45 ) , liver cancer ( 30 , 44 ) , lymphoma ( 6 ) , anaplastic large cell lymphoma ( 6 ) , Coffin-Lowry syndrome ( 46 )
Relevant cell line - cell type - tissue:	'brain, cerebral cortex' ( 15 ) , 3T3 (fibroblast) ( 10 , 25 , 35 ) , bone marrow ( 19 ) , bone marrow [SMAD3 (mouse), homozygous knockout] ( 19 ) , C3H10T1/2 (fibroblast) ( 13 , 39 , 41 ) , CMT-93 (intestinal) ( 45 ) , ES (stem) ( 37 , 42 ) , ESC ( 4 ) , fibroblast ( 46 ) , fibroblast-embryo [MSK1 (mouse), homozygous knockout] ( 46 ) , fibroblast-embryo [MSK2 (mouse), homozygous knockout] ( 46 ) , fibroblast-skin [CDC25A (mouse), transfection] ( 20 ) , germ cell ( 5 ) , GM03189 (lymphoblastoid) ( 44 ) , GM03798 (lymphoblastoid) ( 44 ) , GM04390 (fibroblast) ( 44 ) , granulosa ( 40 ) , HaCaT (keratinocyte) ( 31 ) , HeLa (cervical) ( 39 , 44 ) , Hepa-1 (hepatic) ( 30 ) , hepatocyte-liver ( 8 ) , HepG2 (hepatic) ( 44 ) , HOS (bone cell) ( 21 ) , JB-6 (lymphocyte) ( 6 ) , JB6 CI41 (epidermal) ( 26 , 50 ) , macrophage-bone marrow ( 17 ) , MCF-7 (breast cell) ( 40 ) , MEF (fibroblast) ( 1 , 4 , 6 , 21 , 22 , 25 , 31 , 33 , 34 , 38 , 44 , 47 ) , MEF (fibroblast) [RSK2 (mouse), homozygous knockout] ( 9 ) , oocyte ( 11 , 23 , 27 , 32 ) , ovary ( 40 ) , spermatocytes ( 48 ) , T47D (breast cell) ( 36 )

Upstream Regulation
Regulatory protein:	14-3-3 zeta (mouse) ( 10 ) , AATF (human) ( 35 ) , ATM (mouse) ( 47 ) , ATR (mouse) ( 47 ) , CDC25A (mouse) ( 20 ) , ER-alpha (mouse) ( 40 ) , ER-beta (mouse) ( 40 ) , ERK2 (mouse) ( 50 ) , HDAC3 (mouse) ( 25 ) , HMGN1 (mouse) ( 34 ) , HSF1 (mouse) ( 21 ) , INCENP (mouse) ( 11 ) , Jun (human) ( 22 ) , MAPKAPK2 (human) ( 33 ) , MAPKAPK2 (mouse) ( 33 ) , MAST1 (mouse) ( 1 ) , MSK1 (mouse) ( 37 , 38 ) , MSK2 (mouse) ( 38 ) , P38A (mouse) ( 50 ) , p53 (mouse) ( 33 , 44 ) , RSK2 (mouse) ( 9 ) , SCML2 (mouse) ( 5 ) , SMAD3 (mouse) ( 19 ) , Survivin (mouse) ( 8 )
Putative in vivo kinases:	AurB (human) ( 4 , 8 , 40 ) , AurC (mouse) ( 11 ) , MSK1 (mouse) ( 13 , 26 , 29 , 36 , 37 ) , RSK2 (mouse) ( 31 )
Kinases, in vitro:	AurB (human) ( 2 ) , Chk1 (human) ( 2 ) , ERK2 (mouse) ( 50 ) , MSK1 (mouse) ( 26 , 36 ) , MSK2 (human) ( 2 ) , MST2 (human) ( 2 ) , P38A (human) ( 50 ) , RSK2 (mouse) ( 48 )
Putative upstream phosphatases:	PPP1CB (mouse) ( 32 )
Phosphatases, in vitro:	PPP1CA (mouse) ( 32 ) , PPP2CA (mouse) ( 32 )
Treatments:	adriamycin ( 33 , 44 ) , anisomycin ( 10 , 46 ) , aphidicolin ( 47 ) , bacterial infection ( 17 , 45 ) , benzo(a)pyrene ( 30 ) , caffeine ( 25 ) , chromium ( 30 ) , cisplatin ( 33 ) , colcemid ( 41 ) , EGCG ( 6 ) , EGF ( 6 , 9 , 26 , 31 , 38 ) , estradiol ( 40 ) , H-89 ( 7 , 10 , 37 , 41 ) , IL-1a ( 22 ) , ionizing_radiation ( 20 , 47 ) , kaempferol ( 6 , 31 ) , LIF ( 37 ) , LPS ( 17 , 18 , 45 ) , nocodazole ( 19 , 20 , 34 , 44 ) , okadaic_acid ( 10 , 32 , 48 ) , PD184352 ( 46 ) , PD98059 ( 37 , 50 ) , phorbol_ester ( 13 , 26 , 41 , 46 ) , SB202190 ( 50 ) , SB203580 ( 37 , 46 ) , siRNA ( 33 , 37 ) , TGF-beta ( 45 ) , TNF ( 38 ) , trichostatin_A ( 10 , 45 , 46 ) , U0126 ( 48 ) , UV ( 50 ) , ZM447439 ( 23 )

Downstream Regulation
Effects of modification on H3:	molecular association, regulation ( 13 , 39 )
Effects of modification on biological processes:	cell growth, altered ( 26 ) , chromatin organization, altered ( 13 ) , transcription, altered ( 13 , 17 ) , transcription, induced ( 4 ) , transcription, inhibited ( 7 )
Induce interaction with:	14-3-3 epsilon (mouse) ( 13 ) , 14-3-3 zeta (mouse) ( 13 ) , DNA ( 13 )
Inhibit interaction with:	CBX1 (mouse) ( 39 ) , CBX3 (mouse) ( 39 ) , HP1 alpha (mouse) ( 39 )

References
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