Ser10
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Home > Phosphorylation Site Page: > Ser10  -  H3 (mouse)

Site Information
tkQTARkstGGkAPr   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455676

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 47 ) , immunoassay ( 8 , 11 ) , immunoprecipitation ( 1 , 4 , 10 , 13 ) , mass spectrometry ( 1 , 15 , 39 ) , microscopy-colocalization with upstream kinase ( 4 ) , modification-specific antibody ( 10 , 39 ) , mutation of modification site ( 1 , 20 , 21 , 26 ) , phospho-antibody ( 1 , 4 , 5 , 6 , 8 , 9 , 11 , 13 , 17 , 19 , 21 , 22 , 23 , 25 , 26 , 27 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 50 ) , western blotting ( 1 , 6 , 8 , 9 , 13 , 17 , 19 , 21 , 22 , 23 , 25 , 26 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 40 , 44 , 45 , 46 , 47 , 48 , 50 )
Disease tissue studied:
ataxia-telangiectasia ( 44 ) , bone cancer ( 21 ) , breast cancer ( 36 , 40 ) , colorectal cancer ( 45 ) , colorectal carcinoma ( 45 ) , liver cancer ( 30 , 44 ) , lymphoma ( 6 ) , anaplastic large cell lymphoma ( 6 ) , Coffin-Lowry syndrome ( 46 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 15 ) , 3T3 (fibroblast) ( 10 , 25 , 35 ) , bone marrow ( 19 ) , bone marrow [SMAD3 (mouse), homozygous knockout] ( 19 ) , C3H10T1/2 (fibroblast) ( 13 , 39 , 41 ) , CMT-93 (intestinal) ( 45 ) , ES (stem) ( 37 , 42 ) , ESC ( 4 ) , fibroblast ( 46 ) , fibroblast-embryo [MSK1 (mouse), homozygous knockout] ( 46 ) , fibroblast-embryo [MSK2 (mouse), homozygous knockout] ( 46 ) , fibroblast-skin [CDC25A (mouse), transfection] ( 20 ) , germ cell ( 5 ) , GM03189 (lymphoblastoid) ( 44 ) , GM03798 (lymphoblastoid) ( 44 ) , GM04390 (fibroblast) ( 44 ) , granulosa ( 40 ) , HaCaT (keratinocyte) ( 31 ) , HeLa (cervical) ( 39 , 44 ) , Hepa-1 (hepatic) ( 30 ) , hepatocyte-liver ( 8 ) , HepG2 (hepatic) ( 44 ) , HOS (bone cell) ( 21 ) , JB-6 (lymphocyte) ( 6 ) , JB6 CI41 (epidermal) ( 26 , 50 ) , macrophage-bone marrow ( 17 ) , MCF-7 (breast cell) ( 40 ) , MEF (fibroblast) ( 1 , 4 , 6 , 21 , 22 , 25 , 31 , 33 , 34 , 38 , 44 , 47 ) , MEF (fibroblast) [RSK2 (mouse), homozygous knockout] ( 9 ) , oocyte ( 11 , 23 , 27 , 32 ) , ovary ( 40 ) , spermatocytes ( 48 ) , T47D (breast cell) ( 36 )

Upstream Regulation
Regulatory protein:
14-3-3 zeta (mouse) ( 10 ) , AATF (human) ( 35 ) , ATM (mouse) ( 47 ) , ATR (mouse) ( 47 ) , CDC25A (mouse) ( 20 ) , ER-alpha (mouse) ( 40 ) , ER-beta (mouse) ( 40 ) , ERK2 (mouse) ( 50 ) , HDAC3 (mouse) ( 25 ) , HMGN1 (mouse) ( 34 ) , HSF1 (mouse) ( 21 ) , INCENP (mouse) ( 11 ) , Jun (human) ( 22 ) , MAPKAPK2 (human) ( 33 ) , MAPKAPK2 (mouse) ( 33 ) , MAST1 (mouse) ( 1 ) , MSK1 (mouse) ( 37 , 38 ) , MSK2 (mouse) ( 38 ) , P38A (mouse) ( 50 ) , p53 (mouse) ( 33 , 44 ) , RSK2 (mouse) ( 9 ) , SCML2 (mouse) ( 5 ) , SMAD3 (mouse) ( 19 ) , Survivin (mouse) ( 8 )
Putative in vivo kinases:
AurB (human) ( 4 , 8 , 40 ) , AurC (mouse) ( 11 ) , MSK1 (mouse) ( 13 , 26 , 29 , 36 , 37 ) , RSK2 (mouse) ( 31 )
Kinases, in vitro:
AurB (human) ( 2 ) , Chk1 (human) ( 2 ) , ERK2 (mouse) ( 50 ) , MSK1 (mouse) ( 26 , 36 ) , MSK2 (human) ( 2 ) , MST2 (human) ( 2 ) , P38A (human) ( 50 ) , RSK2 (mouse) ( 48 )
Putative upstream phosphatases:
PPP1CB (mouse) ( 32 )
Phosphatases, in vitro:
PPP1CA (mouse) ( 32 ) , PPP2CA (mouse) ( 32 )
Treatments:
adriamycin ( 33 , 44 ) , anisomycin ( 10 , 46 ) , aphidicolin ( 47 ) , bacterial infection ( 17 , 45 ) , benzo(a)pyrene ( 30 ) , caffeine ( 25 ) , chromium ( 30 ) , cisplatin ( 33 ) , colcemid ( 41 ) , EGCG ( 6 ) , EGF ( 6 , 9 , 26 , 31 , 38 ) , estradiol ( 40 ) , H-89 ( 7 , 10 , 37 , 41 ) , IL-1a ( 22 ) , ionizing_radiation ( 20 , 47 ) , kaempferol ( 6 , 31 ) , LIF ( 37 ) , LPS ( 17 , 18 , 45 ) , nocodazole ( 19 , 20 , 34 , 44 ) , okadaic_acid ( 10 , 32 , 48 ) , PD184352 ( 46 ) , PD98059 ( 37 , 50 ) , phorbol_ester ( 13 , 26 , 41 , 46 ) , SB202190 ( 50 ) , SB203580 ( 37 , 46 ) , siRNA ( 33 , 37 ) , TGF-beta ( 45 ) , TNF ( 38 ) , trichostatin_A ( 10 , 45 , 46 ) , U0126 ( 48 ) , UV ( 50 ) , ZM447439 ( 23 )

Downstream Regulation
Effects of modification on H3:
molecular association, regulation ( 13 , 39 )
Effects of modification on biological processes:
cell growth, altered ( 26 ) , chromatin organization, altered ( 13 ) , transcription, altered ( 13 , 17 ) , transcription, induced ( 4 ) , transcription, inhibited ( 7 )
Induce interaction with:
14-3-3 epsilon (mouse) ( 13 ) , 14-3-3 zeta (mouse) ( 13 ) , DNA ( 13 )
Inhibit interaction with:
CBX1 (mouse) ( 39 ) , CBX3 (mouse) ( 39 ) , HP1 alpha (mouse) ( 39 )

References 

1

Diril MK, et al. (2016) Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet 12, e1006310
27631493   Curated Info

2

Stützer A, et al. (2016) Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails. Mol Cell 61, 247-59
26778125   Curated Info

3

Sidoli S, et al. (2015) Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH) Analysis for Characterization and Quantification of Histone Post-translational Modifications. Mol Cell Proteomics 14, 2420-8
25636311   Curated Info

4

Mallm JP, Rippe K (2015) Aurora Kinase B Regulates Telomerase Activity via a Centromeric RNA in Stem Cells. Cell Rep 11, 1667-78
26051938   Curated Info

5

Hasegawa K, et al. (2015) SCML2 Establishes the Male Germline Epigenome through Regulation of Histone H2A Ubiquitination. Dev Cell 32, 574-88
25703348   Curated Info

6

Chen H, et al. (2015) Computational and Biochemical Discovery of RSK2 as a Novel Target for Epigallocatechin Gallate (EGCG). PLoS One 10, e0130049
26083344   Curated Info

7

Sabbattini P, et al. (2014) An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation. Mol Biol Cell 25, 904-15
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8

Hagemann S, et al. (2013) Loss of Survivin influences liver regeneration and is associated with impaired Aurora B function. Cell Death Differ 20, 834-44
23519077   Curated Info

9

Lau AT, et al. (2011) Phosphorylation of histone H2B serine 32 is linked to cell transformation. J Biol Chem 286, 26628-37
21646345   Curated Info

10

Simboeck E, et al. (2010) A phosphorylation switch regulates the transcriptional activation of cell cycle regulator p21 by histone deacetylase inhibitors. J Biol Chem 285, 41062-73
20952396   Curated Info

11

Yang KT, et al. (2010) Aurora-C kinase deficiency causes cytokinesis failure in meiosis I and production of large polyploid oocytes in mice. Mol Biol Cell 21, 2371-83
20484572   Curated Info

12

Sayyed SG, et al. (2010) Progressive glomerulosclerosis in type 2 diabetes is associated with renal histone H3K9 and H3K23 acetylation, H3K4 dimethylation and phosphorylation at serine 10. Nephrol Dial Transplant 25, 1811-7
20067909   Curated Info

13

Drobic B, et al. (2010) Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex. Nucleic Acids Res 38, 3196-208
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14

Rajendrasozhan S, et al. (2010) Targeted disruption of NF-{kappa}B1 (p50) augments cigarette smoke-induced lung inflammation and emphysema in mice: a critical role of p50 in chromatin remodeling. Am J Physiol Lung Cell Mol Physiol 298, L197-209
19965984   Curated Info

15

Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82
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16

Colussi C, et al. (2009) Nitric oxide deficiency determines global chromatin changes in Duchenne muscular dystrophy. FASEB J 23, 2131-41
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17

Leng J, et al. (2009) Toxoplasma gondii prevents chromatin remodeling initiated by TLR-triggered macrophage activation. J Immunol 182, 489-97
19109180   Curated Info

18

Leng J, Denkers EY (2009) Toxoplasma gondii inhibits covalent modification of histone H3 at the IL-10 promoter in infected macrophages. PLoS One 4, e7589
19859561   Curated Info

19

Fujita T, et al. (2008) Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells. Mol Biol Cell 19, 5446-55
18843049   Curated Info

20

Shreeram S, Hee WK, Bulavin DV (2008) Cdc25A serine 123 phosphorylation couples centrosome duplication with DNA replication and regulates tumorigenesis. Mol Cell Biol 28, 7442-50
18936171   Curated Info

21

Lee YJ, et al. (2008) HSF1 as a mitotic regulator: phosphorylation of HSF1 by Plk1 is essential for mitotic progression. Cancer Res 68, 7550-60
18794143   Curated Info

22

Wolter S, et al. (2008) c-Jun controls histone modifications, NF-kappaB recruitment, and RNA polymerase II function to activate the ccl2 gene. Mol Cell Biol 28, 4407-23
18443042   Curated Info

23

Swain JE, Ding J, Wu J, Smith GD (2008) Regulation of spindle and chromatin dynamics during early and late stages of oocyte maturation by aurora kinases. Mol Hum Reprod 14, 291-9
18353803   Curated Info

24

Gwinn DM, et al. (2008) AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell 30, 214-26
18439900   Curated Info

25

Bhaskara S, et al. (2008) Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Mol Cell 30, 61-72
18406327   Curated Info

26

Kim HG, et al. (2008) Mitogen- and stress-activated kinase 1-mediated histone H3 phosphorylation is crucial for cell transformation. Cancer Res 68, 2538-47
18381464   Curated Info

27

Wang Q, et al. (2008) The spatial relationship between heterochromatin protein 1 alpha and histone modifications during mouse oocyte meiosis. Cell Cycle 7, 513-20
18239470   Curated Info

28

Shimada M, et al. (2008) Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression. Cell 132, 221-32
18243098   Curated Info

29

Basu S, et al. (2007) Mycobacterium avium-induced matrix metalloproteinase-9 expression occurs in a cyclooxygenase-2-dependent manner and involves phosphorylation- and acetylation-dependent chromatin modification. Cell Microbiol 9, 2804-16
17590163   Curated Info

30

Schnekenburger M, Talaska G, Puga A (2007) Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation. Mol Cell Biol 27, 7089-101
17682057   Curated Info

31

Cho YY, et al. (2007) Ribosomal S6 kinase 2 is a key regulator in tumor promoter induced cell transformation. Cancer Res 67, 8104-12
17804722   Curated Info

32

Swain JE, et al. (2007) Proper chromatin condensation and maintenance of histone H3 phosphorylation during mouse oocyte meiosis requires protein phosphatase activity. Biol Reprod 76, 628-38
17182892   Curated Info

33

Reinhardt HC, Aslanian AS, Lees JA, Yaffe MB (2007) p53-deficient cells rely on ATM- and ATR-mediated checkpoint signaling through the p38MAPK/MK2 pathway for survival after DNA damage. Cancer Cell 11, 175-89
17292828   Curated Info

34

Postnikov YV, Belova GI, Lim JH, Bustin M (2006) Chromosomal protein HMGN1 modulates the phosphorylation of serine 1 in histone H2A. Biochemistry 45, 15092-9
17154547   Curated Info

35

Bruno T, et al. (2006) Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell 10, 473-86
17157788   Curated Info

36

Vicent GP, et al. (2006) Induction of progesterone target genes requires activation of Erk and Msk kinases and phosphorylation of histone H3. Mol Cell 24, 367-81
17081988   Curated Info

37

Lee ER, McCool KW, Murdoch FE, Fritsch MK (2006) Dynamic changes in histone H3 phosphoacetylation during early embryonic stem cell differentiation are directly mediated by mitogen- and stress-activated protein kinase 1 via activation of MAPK pathways. J Biol Chem 281, 21162-72
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38

Duncan EA, Anest V, Cogswell P, Baldwin AS (2006) The kinases MSK1 and MSK2 are required for epidermal growth factor-induced, but not tumor necrosis factor-induced, histone H3 Ser10 phosphorylation. J Biol Chem 281, 12521-5
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39

Fischle W, et al. (2005) Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation. Nature 438, 1116-22
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40

Ruiz-Cortés ZT, et al. (2005) Estrogen mediates phosphorylation of histone H3 in ovarian follicle and mammary epithelial tumor cells via the mitotic kinase, Aurora B. Mol Endocrinol 19, 2991-3000
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41

Dunn KL, Davie JR (2005) Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28. Oncogene 24, 3492-502
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42

Illi B, et al. (2005) Epigenetic histone modification and cardiovascular lineage programming in mouse embryonic stem cells exposed to laminar shear stress. Circ Res 96, 501-8
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43

Polioudaki H, et al. (2004) Mitotic phosphorylation of histone H3 at threonine 3. FEBS Lett 560, 39-44
14987995   Curated Info

44

Chow JP, et al. (2003) DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase. Mol Biol Cell 14, 3989-4002
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45

Haller D, et al. (2003) Transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation. J Biol Chem 278, 23851-60
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46

Soloaga A, et al. (2003) MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14. EMBO J 22, 2788-97
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47

Brown EJ, Baltimore D (2003) Essential and dispensable roles of ATR in cell cycle arrest and genome maintenance. Genes Dev 17, 615-28
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48

Di Agostino S, Rossi P, Geremia R, Sette C (2002) The MAPK pathway triggers activation of Nek2 during chromosome condensation in mouse spermatocytes. Development 129, 1715-27
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49

Lachner M, et al. (2001) Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Nature 410, 116-20
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50

Zhong SP, Ma WY, Dong Z (2000) ERKs and p38 kinases mediate ultraviolet B-induced phosphorylation of histone H3 at serine 10. J Biol Chem 275, 20980-4
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