Tyr685
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Home > Phosphorylation Site Page: > Tyr685  -  CDH5 (mouse)

Site Information
MDARPAVyTQVQkPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455658
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 , 4 ) , mass spectrometry ( 5 , 6 , 7 , 9 , 10 , 11 ) , mutation of modification site ( 4 , 8 , 12 ) , phospho-antibody ( 2 , 3 , 4 ) , phosphopeptide mapping ( 8 ) , western blotting ( 2 , 3 , 4 , 8 )
Disease tissue studied:
lung cancer ( 7 )
Relevant cell line - cell type - tissue:
'brain, embryonic' ( 10 ) , cardiac-heart ( 2 ) , CHO (fibroblast) ( 8 , 12 ) , COS (fibroblast) ( 12 ) , endothelial [CDH5 (mouse), genetic knockin] ( 4 ) , heart ( 9 ) , HLMVECs ( 3 ) , leukocyte [CDH5 (mouse), genetic knockin] ( 4 ) , lung ( 3 , 5 , 11 ) , lung [HRas (mouse), transgenic] ( 7 ) , microvessel endothelial-'brain, forebrain' ( 8 )

Upstream Regulation
Regulatory protein:
ANXA2 (mouse) ( 2 )
Kinases, in vitro:
Src (human) ( 12 )
Putative upstream phosphatases:
PTPRB (mouse) ( 4 )
Treatments:
histamine ( 4 ) , PF-431396 ( 3 ) , PP1 ( 3 ) , vanadate ( 4 ) , VEGF ( 2 , 4 )

Downstream Regulation
Effects of modification on CDH5:
molecular association, regulation ( 12 ) , phosphorylation ( 12 )
Effects of modification on biological processes:
cell growth, altered ( 12 )
Induce interaction with:
CSK (human) ( 12 )

References 

1

Fu P, et al. (2020) Phospholipase D2 restores endothelial barrier function by promoting PTPN14-mediated VE-cadherin dephosphorylation. J Biol Chem
32327488   Curated Info

2

Luo M, et al. (2017) Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases. J Exp Med 214, 2535-2545
28694388   Curated Info

3

Soni D, et al. (2017) Pyk2 phosphorylation of VE-PTP downstream of STIM1-induced Ca(2+) entry regulates disassembly of adherens junctions. Am J Physiol Lung Cell Mol Physiol 312, L1003-L1017
28385807   Curated Info

4

Wessel F, et al. (2014) Leukocyte extravasation and vascular permeability are each controlled in vivo by different tyrosine residues of VE-cadherin. Nat Immunol 15, 223-30
24487320   Curated Info

5

Rikova K (2008) CST Curation Set: 4985; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Rikova K (2008) CST Curation Set: 4837; Year: 2008; Biosample/Treatment: cell line, Dana Farber-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Ren H (2008) CST Curation Set: 4337; Year: 2008; Biosample/Treatment: cell line, Ras_WT/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Turowski P, et al. (2008) Phosphorylation of vascular endothelial cadherin controls lymphocyte emigration. J Cell Sci 121, 29-37
18096689   Curated Info

9

Guo A (2007) CST Curation Set: 2827; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Guo A (2007) CST Curation Set: 2672; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Farnsworth C (2006) CST Curation Set: 2094; Year: 2006; Biosample/Treatment: tissue, lung/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Baumeister U, et al. (2005) Association of Csk to VE-cadherin and inhibition of cell proliferation. EMBO J 24, 1686-95
15861137   Curated Info