Thr977
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Home > Phosphorylation Site Page: > Thr977  -  Met (human)

Site Information
RyDARVHtPHLDRLV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2192573

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 )
Disease tissue studied:
luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 ) , cervical cancer ( 9 ) , cervical adenocarcinoma ( 9 ) , colorectal cancer ( 12 ) , colorectal carcinoma ( 12 ) , lung cancer ( 3 , 12 ) , non-small cell lung adenocarcinoma ( 3 )
Relevant cell line - cell type - tissue:
A498 (renal) ( 8 ) , A549 (pulmonary) ( 4 , 12 ) , breast ( 2 ) , HCT116 (intestinal) ( 12 ) , HeLa (cervical) ( 1 , 6 ) , HeLa S3 (cervical) ( 9 , 11 ) , HeLa_Meta (cervical) ( 7 ) , HeLa_Pro (cervical) ( 7 ) , HeLa_Telo (cervical) ( 7 ) , MDA-MB-435S (breast cell) ( 10 ) , PC9 (pulmonary) ( 3 ) , PC9-IR (pulmonary) ( 3 )

Upstream Regulation
Treatments:
EGF ( 1 ) , LPA ( 8 ) , MG132_withdrawal ( 7 ) , nocodazole ( 9 , 11 ) , thymidine ( 11 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

4

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

8

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

9

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

10

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

11

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

12

Wissing J, et al. (2007) Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. Mol Cell Proteomics 6, 537-47
17192257   Curated Info