Lys627

Site Information
ERDGLKEkVYQMLQk SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2188102

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 4 ) , modification-specific antibody ( 4 ) , multiple reaction monitoring (MRM) ( 4 ) , mutation of modification site ( 1 ) , western blotting ( 1 , 4 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 4 ) , HEK293T (epithelial) ( 1 , 4 ) , HEp-2 (squamous) ( 2 ) , Jurkat (T lymphocyte) ( 2 ) , macrophage-bone marrow ( 1 ) , MEF (fibroblast) ( 1 )

Downstream Regulation
Effects of modification on RIPK1:	enzymatic activity, induced ( 1 ) , ubiquitination ( 1 )
Effects of modification on biological processes:	apoptosis, induced ( 1 )
Induce interaction with:	CASP8 (human) ( 1 ) , FADD (human) ( 1 )

References
1	Li X, et al. (2020) Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun 11, 6364 33311474 Curated Info
2	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
3	Feltham R, et al. (2018) Mind Bomb Regulates Cell Death during TNF Signaling by Suppressing RIPK1's Cytotoxic Potential. Cell Rep 29642005 Curated Info
4	Mollah S, et al. (2007) Targeted mass spectrometric strategy for global mapping of ubiquitination on proteins. Rapid Commun Mass Spectrom 21, 3357-64 17883243 Curated Info