Ser2030
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Home > Phosphorylation Site Page: > Ser2030  -  RYR2 (mouse)

Site Information
TIRGRLLsLVEkVTY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455603

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 4 ) , mutation of modification site ( 2 , 5 , 6 , 8 ) , phospho-antibody ( 1 , 2 , 7 , 8 ) , western blotting ( 1 , 2 , 8 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 , 6 , 7 , 8 ) , heart ( 1 , 3 , 4 , 8 ) , myocardium ( 2 ) , myocyte-heart ( 2 , 7 , 8 )

Upstream Regulation
Regulatory protein:
PKACA (human) ( 2 ) , plakophilin 2 (mouse) ( 1 )
Putative in vivo kinases:
PKACA (human) ( 7 , 8 )
Kinases, in vitro:
PKACA (human) ( 6 , 7 , 8 )
Treatments:
heart failure ( 8 ) , isoproterenol ( 2 , 7 , 8 ) , mPKI ( 7 ) , okadaic_acid ( 2 ) , wheel running test ( 1 )

Downstream Regulation
Effects of modification on RYR2:
activity, induced ( 2 , 6 ) , phosphorylation ( 2 ) , protein conformation ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
hypertrophic cardiomyopathy ( 2 )

References 

1

Hammer KP, et al. (2021) Beneficial effect of voluntary physical exercise in Plakophilin2 transgenic mice. PLoS One 16, e0252649
34086773   Curated Info

2

Potenza DM, et al. (2018) Phosphorylation of the ryanodine receptor 2 at serine 2030 is required for a complete β-adrenergic response. J Gen Physiol
30541771   Curated Info

3

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

4

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

5

Jones PP, et al. (2008) Localization of PKA phosphorylation site, Ser(2030), in the three-dimensional structure of cardiac ryanodine receptor. Biochem J 410, 261-70
17967164   Curated Info

6

Xiao B, et al. (2007) Functional consequence of protein kinase A-dependent phosphorylation of the cardiac ryanodine receptor: sensitization of store overload-induced Ca2+ release. J Biol Chem 282, 30256-64
17693412   Curated Info

7

Xiao B, et al. (2006) Ser-2030, but not Ser-2808, is the major phosphorylation site in cardiac ryanodine receptors responding to protein kinase A activation upon beta-adrenergic stimulation in normal and failing hearts. Biochem J 396, 7-16
16483256   Curated Info

8

Xiao B, et al. (2005) Characterization of a novel PKA phosphorylation site, serine-2030, reveals no PKA hyperphosphorylation of the cardiac ryanodine receptor in canine heart failure. Circ Res 96, 847-55
15790957   Curated Info