Ser664
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Home > Phosphorylation Site Page: > Ser664  -  TSC2 (human)

Site Information
KkTSGPLsPPTGPPG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455575

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 11 ) , mass spectrometry ( 2 , 4 , 5 , 7 , 9 , 11 , 12 ) , mutation of modification site ( 10 , 11 ) , peptide sequencing ( 2 ) , phospho-antibody ( 1 , 2 , 3 , 8 , 10 , 12 ) , western blotting ( 1 , 2 , 3 , 10 , 12 )
Disease tissue studied:
breast cancer ( 4 , 10 ) , breast ductal carcinoma ( 4 ) , colorectal cancer ( 3 , 10 ) , colorectal carcinoma ( 3 ) , endometrial cancer ( 10 ) , kidney cancer ( 10 ) , leukemia ( 9 ) , chronic myelogenous leukemia ( 9 ) , tuberous sclerosis ( 10 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 10 ) , 'muscle, skeletal' ( 2 ) , 293 (epithelial) ( 11 ) , 293E (epithelial) ( 12 ) , breast ( 4 ) , breast cell-breast ( 10 ) , Caco-2 (intestinal) ( 3 ) , colon ( 10 ) , endometrium ( 10 ) , HCT116 (intestinal) ( 3 ) , HEK293T (epithelial) ( 1 , 10 , 11 ) , HeLa (cervical) ( 7 ) , Jurkat (T lymphocyte) ( 5 ) , K562 (erythroid) ( 7 , 9 ) , kidney ( 10 ) , lung ( 8 ) , MDA-MB-231 (breast cell) ( 10 ) , NCI-H1650 (pulmonary) ( 10 ) , RKO (intestinal) ( 3 ) , SK-MEL2 (melanocyte) ( 10 ) , SW480 (intestinal) ( 3 ) , SW620 (intestinal) ( 3 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (human) ( 10 ) , ERK2 (human) ( 10 , 11 )
Treatments:
eccentric contractions ( 2 ) , phorbol_ester ( 10 , 12 ) , SB202190 ( 3 ) , U0126 ( 10 )

Downstream Regulation
Effects of modification on TSC2:
activity, induced ( 8 ) , enzymatic activity, induced ( 8 ) , molecular association, regulation ( 11 )
Inhibit interaction with:
TSC1 (human) ( 11 )

Disease / Diagnostics Relevance
Relevant diseases:
tuberous sclerosis ( 10 )

References 

1

Romero-Pozuelo J, et al. (2020) Cdk4 and Cdk6 Couple the Cell-Cycle Machinery to Cell Growth via mTORC1. Cell Rep 31, 107504
32294430   Curated Info

2

Jacobs BL, et al. (2017) Identification of mechanically regulated phosphorylation sites on tuberin (TSC2) that control mechanistic target of rapamycin (mTOR) signaling. J Biol Chem 292, 6987-6997
28289099   Curated Info

3

Zhang Y, et al. (2015) PP2AC Level Determines Differential Programming of p38-TSC-mTOR Signaling and Therapeutic Response to p38-Targeted Therapy in Colorectal Cancer. EBioMedicine 2, 1944-56
26844273   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

7

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

8

Yoshizawa A, et al. (2010) Overexpression of phospho-eIF4E is associated with survival through AKT pathway in non-small cell lung cancer. Clin Cancer Res 16, 240-8
20008839   Curated Info

9

Moritz A (2009) CST Curation Set: 8425; Year: 2009; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Ma L, et al. (2007) Identification of S664 TSC2 phosphorylation as a marker for extracellular signal-regulated kinase mediated mTOR activation in tuberous sclerosis and human cancer. Cancer Res 67, 7106-12
17671177   Curated Info

11

Ma L, et al. (2005) Phosphorylation and functional inactivation of TSC2 by Erk implications for tuberous sclerosis and cancer pathogenesis. Cell 121, 179-93
15851026   Curated Info

12

Ballif BA, et al. (2005) Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors. Proc Natl Acad Sci U S A 102, 667-72
15647351   Curated Info