Thr641
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr641  -  NCL (human)

Site Information
EIDGNkVtLDWAkPK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 21697920

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 , 2 ) , mass spectrometry ( 3 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Disease tissue studied:
lung cancer ( 2 ) , non-small cell lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
A431 (epithelial) ( 1 ) , A549 (pulmonary) ( 2 ) , E.coli (bacterial) ( 2 ) , HeLa (cervical) ( 1 , 2 ) , IMR-90 (fibroblast) ( 2 ) , Jurkat (T lymphocyte) ( 3 ) , lung ( 2 )

Upstream Regulation
Putative in vivo kinases:
Akt1 (human) ( 1 ) , CDK1 (human) ( 2 )
Kinases, in vitro:
CDK1 (human) ( 2 )
Treatments:
EGF ( 1 ) , gefitinib ( 1 ) , LY294002 ( 1 ) , MK-2206 ( 1 ) , siRNA ( 2 ) , U0126 ( 1 )

Downstream Regulation
Effects of modification on NCL:
molecular association, regulation ( 1 ) , protein stabilization ( 1 , 2 )
Effects of modification on biological processes:
carcinogenesis, induced ( 2 ) , cell growth, induced ( 1 ) , RNA stability, induced ( 2 ) , translation, induced ( 1 )
Induce interaction with:
RNA ( 1 )

Disease / Diagnostics Relevance
Relevant diseases:
lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 1 )

References 

1

Hung CY, et al. (2014) Nucleolin enhances internal ribosomal entry site (IRES)-mediated translation of Sp1 in tumorigenesis. Biochim Biophys Acta 1843, 2843-54
25173817   Curated Info

2

Wang SA, et al. (2011) Heat shock protein 90 stabilizes nucleolin to increase mRNA stability in mitosis. J Biol Chem 286, 43816-29
21998300   Curated Info

3

Guo A (2011) CST Curation Set: 12018; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.