Ser164
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Home > Phosphorylation Site Page: > Ser164  -  TOB1 (human)

Site Information
FGHSAAVsPTFMPRS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455537

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , mutation of modification site ( 3 , 4 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 )
Disease tissue studied:
breast cancer ( 1 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 3 , 4 ) , MCF-7 (breast cell) ( 1 ) , MEF (fibroblast) [IGF1R (mouse)] ( 4 )

Upstream Regulation
Regulatory protein:
TOB1 (human) ( 1 )
Putative in vivo kinases:
ERK1 (human) ( 4 ) , ERK2 (human) ( 3 , 4 )
Kinases, in vitro:
ERK1 (human) ( 4 ) , ERK2 (human) ( 3 , 4 ) , JNK2 (human) ( 3 )
Treatments:
AG1296 ( 4 ) , EGF ( 3 , 4 ) , estradiol ( 3 ) , FGF1 ( 3 ) , PD98059 ( 4 ) , PDGF ( 3 , 4 ) , serum ( 3 , 4 ) , U0126 ( 3 )

Downstream Regulation
Effects of modification on TOB1:
activity, inhibited ( 3 , 4 )
Effects of modification on biological processes:
cell cycle regulation ( 3 ) , cell growth, altered ( 4 )

References 

1

Zhang YW, et al. (2016) Acquisition of estrogen independence induces TOB1-related mechanisms supporting breast cancer cell proliferation. Oncogene 35, 1643-56
26165839   Curated Info

2

Ezzeddine N, Chen CY, Shyu AB (2012) Evidence Providing New Insights into TOB-Promoted Deadenylation and Supporting a Link between TOB's Deadenylation-Enhancing and Antiproliferative Activities. Mol Cell Biol 32, 1089-98
22252318   Curated Info

3

Maekawa M, Nishida E, Tanoue T (2002) Identification of the Anti-proliferative protein Tob as a MAPK substrate. J Biol Chem 277, 37783-7
12151396   Curated Info

4

Suzuki T, et al. (2002) Phosphorylation of three regulatory serines of Tob by Erk1 and Erk2 is required for Ras-mediated cell proliferation and transformation. Genes Dev 16, 1356-70
12050114   Curated Info