Thr402
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Home > Phosphorylation Site Page: > Thr402  -  CDT1 (human)

Site Information
PrPALPAtPPAtPPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 20804634

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 1 ) , immunoprecipitation ( 5 ) , mass spectrometry ( 2 , 3 , 6 , 7 , 8 ) , mutation of modification site ( 1 , 5 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Disease tissue studied:
bone cancer ( 1 ) , colorectal cancer ( 5 ) , colorectal carcinoma ( 5 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 5 ) , fibroblast ( 5 ) , HCT116 (intestinal) ( 5 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 1 , 2 , 5 ) , Jurkat (T lymphocyte) ( 3 , 6 , 7 , 8 ) , U2OS (bone cell) ( 1 )

Upstream Regulation
Putative in vivo kinases:
JNK1 (human) ( 5 ) , P38A (human) ( 5 )
Kinases, in vitro:
CDK1 (human) ( 1 ) , P38A (human) ( 5 )
Treatments:
calyculin_A ( 1 ) , CVT-313 ( 1 ) , JNK_inhibitor_VIII ( 1 ) , nocodazole ( 1 , 5 ) , okadaic_acid ( 1 ) , RO-3306 ( 1 ) , SB203580 ( 1 , 5 ) , sorbitol ( 5 ) , UV ( 5 )

Downstream Regulation
Effects of modification on CDT1:
activity, inhibited ( 5 ) , molecular association, regulation ( 1 , 5 ) , phosphorylation ( 1 ) , protein stabilization ( 5 )
Inhibit interaction with:
MCM2 (human) ( 1 ) , RAMP (human) ( 5 )

References 

1

Zhou Y, et al. (2020) Distinct and sequential re-replication barriers ensure precise genome duplication. PLoS Genet 16, e1008988
32841231   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Chandrasekaran S, Tan TX, Hall JR, Cook JG (2011) Stress-stimulated mitogen-activated protein kinases control the stability and activity of the cdt1 DNA replication licensing factor. Mol Cell Biol 31, 4405-16
21930785   Curated Info

6

Possemato A (2008) CST Curation Set: 5273; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

7

Possemato A (2008) CST Curation Set: 5275; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

8

Stokes M (2008) CST Curation Set: 3882; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info