Ser422

Site Information
AHPPHAPsPGQTVkP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 455350

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 ) , mutation of modification site ( 5 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 4 )
Disease tissue studied:	bone cancer ( 4 ) , osteosarcoma ( 4 ) , lung cancer ( 4 )
Relevant cell line - cell type - tissue:	COS (fibroblast) ( 3 , 4 ) , HeLa (cervical) ( 1 , 4 ) , liver ( 2 ) , MEF (fibroblast) [CTBP1 (mouse), homozygous knockout] ( 5 ) , NCI-H1299 (pulmonary) ( 3 , 4 ) , U2OS (bone cell) ( 4 )

Downstream Regulation
Effects of modification on CTBP1:	protein degradation ( 3 , 4 , 5 )
Effects of modification on biological processes:	apoptosis, induced ( 3 , 5 )

References
1	Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296 27281782 Curated Info
2	Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62 24275569 Curated Info
3	Wang SY, Iordanov M, Zhang Q (2006) c-Jun NH2-terminal kinase promotes apoptosis by down-regulating the transcriptional co-repressor CtBP. J Biol Chem 281, 34810-5 16984892 Curated Info
4	Zhang Q, Nottke A, Goodman RH (2005) Homeodomain-interacting protein kinase-2 mediates CtBP phosphorylation and degradation in UV-triggered apoptosis. Proc Natl Acad Sci U S A 102, 2802-7 15708980 Curated Info
5	Zhang Q, et al. (2003) Homeodomain interacting protein kinase 2 promotes apoptosis by downregulating the transcriptional corepressor CtBP. Cell 115, 177-86 14567915 Curated Info