Lys139

Site Information
VMGVNHEkyDNsLkI SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 21175144

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:	liver cancer ( 1 ) , liver adenocarcinoma ( 1 ) , hepatocellular carcinoma ( 1 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 1 ) , HEp-2 (squamous) ( 2 ) , HepG2 (hepatic) ( 1 ) , Huh7 (hepatic) ( 1 ) , Jurkat (T lymphocyte) ( 2 , 3 , 4 ) , SK-HEP-1 (endothelial) ( 1 ) , U2OS (bone cell) ( 5 )

Downstream Regulation
Effects of modification on GAPDH:	activity, induced ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:	cell growth, induced ( 1 ) , cell motility, induced ( 1 )
Induce interaction with:	TRAF2 (human) ( 1 )

References
1	Lin XT, et al. (2023) Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice. Cell Rep 42, 112812 37450367 Curated Info
2	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
3	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
4	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info
5	Povlsen LK, et al. (2012) Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. Nat Cell Biol 14, 1089-98 23000965 Curated Info
6	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info