Ser15

Site Information
FSLLRSPsWEPFRDW SwissProt
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448401

In vivo Characterization
Methods used to characterize site in vivo:	mutation of modification site ( 1 , 2 , 3 , 4 , 5 ) , phospho-antibody ( 2 , 3 ) , western blotting ( 2 , 3 )
Disease tissue studied:	colorectal cancer ( 2 ) , colorectal carcinoma ( 2 )
Relevant cell line - cell type - tissue:	3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 4 ) , 3T3 (fibroblast) ( 5 ) , Caco-2 (intestinal) ( 2 ) , E.coli (bacterial) ( 1 ) , LLC-PK1 (renal) ( 3 ) , T84 (intestinal) ( 2 )

Downstream Regulation
Effects of modification on HSP27:	activity, induced ( 4 ) , molecular association, regulation ( 2 ) , protein conformation ( 1 )
Effects of modification on biological processes:	apoptosis, altered ( 3 )
Induce interaction with:	IKKA (human) ( 2 )
Inhibit interaction with:	HSP27 (human) ( 1 )

References
1	Sha E, et al. (2019) Functional and structural characterization of HspB1/Hsp27 from Chinese hamster ovary cells. FEBS Open Bio 31441240 Curated Info
2	Kammanadiminti SJ, Chadee K (2006) Suppression of NF-kappaB activation by Entamoeba histolytica in intestinal epithelial cells is mediated by heat shock protein 27. J Biol Chem 281, 26112-20 16840786 Curated Info
3	de Graauw M, et al. (2005) Heat shock protein 27 is the major differentially phosphorylated protein involved in renal epithelial cellular stress response and controls focal adhesion organization and apoptosis. J Biol Chem 280, 29885-98 15944157 Curated Info
4	Thériault JR, et al. (2004) Essential role of the NH2-terminal WD/EPF motif in the phosphorylation-activated protective function of mammalian Hsp27. J Biol Chem 279, 23463-71 15033973 Curated Info
5	Lambert H, et al. (1999) HSP27 multimerization mediated by phosphorylation-sensitive intermolecular interactions at the amino terminus. J Biol Chem 274, 9378-85 10092617 Curated Info