Ser231

Site Information
RPMRKsFsQPGLRSL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2103609
Associated spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 4 , 7 , 8 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ) , mutation of modification site ( 2 ) , phospho-antibody ( 1 , 2 , 3 , 6 ) , western blotting ( 1 , 2 , 3 , 6 )
Disease tissue studied:	anthrax infection ( 22 ) , neuroblastoma ( 21 ) , melanoma skin cancer ( 32 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 12 ) , 'fat, brown' ( 23 ) , 'muscle, skeletal' ( 3 , 6 , 33 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 31 ) , 32Dcl3 (myeloid) ( 31 ) , brain ( 17 , 18 , 23 , 24 , 26 , 28 , 35 ) , C2C12 (myoblast) ( 30 ) , heart ( 1 , 13 ) , hepatocyte-liver ( 2 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 8 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 8 ) , HL-1 (myocyte) ( 8 ) , kidney ( 23 ) , L6 (myoblast) ( 6 ) , liver ( 4 , 11 , 16 , 25 , 27 ) , lung ( 23 ) , macrophage-peritoneum ( 15 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 19 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 14 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 14 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 20 ) , MEF (fibroblast) ( 14 ) , mpkCCD (renal) ( 29 ) , N1E-115 (neuron) ( 21 ) , pancreas ( 23 ) , RAW 264.7 (macrophage) ( 7 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 32 ) , spleen ( 22 , 23 ) , testis ( 23 )

Upstream Regulation
Regulatory protein:	RIPK3 (mouse) ( 15 ) , TLR9 (human) ( 3 )
Putative in vivo kinases:	AMPKA1 (mouse) ( 33 ) , AMPKA2 (human) ( 2 )
Kinases, in vitro:	Akt1 (human) ( 33 ) , AMPKA1 (human) ( 33 )
Treatments:	acadesine ( 33 ) , electrical_stimulation ( 3 ) , exercise ( 3 , 10 ) , G-1 ( 1 ) , high-fat diet ( 1 ) , insulin ( 10 , 12 , 33 ) , LPA ( 21 ) , LY294002 ( 12 ) , MSH ( 6 ) , muscle contraction ( 10 , 33 ) , obesity-inducing diet ( 2 ) , PF739 ( 3 )

Downstream Regulation
Effects of modification on biological processes:	signaling pathway regulation ( 1 ) , transcription, inhibited ( 2 )

References
1	Lu C, et al. (2024) GPR30 selective agonist G-1 induced insulin resistance in ovariectomized mice on high fat diet and its mechanism. Biochem Biophys Res Commun 716, 150026 38701557 Curated Info
2	Chen ZY, et al. (2022) Rab2A regulates the progression of nonalcoholic fatty liver disease downstream of AMPK-TBC1D1 axis by stabilizing PPARγ. PLoS Biol 20, e3001522 35061665 Curated Info
3	Liu Y, et al. (2020) TLR9 and beclin¿¿1 crosstalk regulates muscle AMPK activation in exercise. Nature 32051584 Curated Info
4	Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127 27818261 Curated Info
5	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
6	Møller CL, et al. (2016) α-MSH Stimulates Glucose Uptake in Mouse Muscle and Phosphorylates Rab-GTPase-Activating Protein TBC1D1 Independently of AMPK. PLoS One 11, e0157027 27467141 Curated Info
7	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
8	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
9	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
10	Treebak JT, et al. (2014) Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol 592, 351-75 24247980 Curated Info
11	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
12	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
13	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
14	Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3 23429704 Curated Info
15	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
16	Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83 23140645 Curated Info
17	Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29 22645316 Curated Info
18	Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9 22807455 Curated Info
19	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
20	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
21	Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201 21454597 Curated Info
22	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
23	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
24	Possemato A (2010) CST Curation Set: 9879; Year: 2010; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb Cat#: 9606 Curated Info
25	Possemato A (2010) CST Curation Set: 9883; Year: 2010; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601 Curated Info
26	Possemato A (2010) CST Curation Set: 9878; Year: 2010; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb Cat#: 9606 Curated Info
27	Possemato A (2010) CST Curation Set: 9882; Year: 2010; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601 Curated Info
28	Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9 20415495 Curated Info
29	Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7 20139300 Curated Info
30	Peck GR, et al. (2009) Insulin-stimulated phosphorylation of the Rab GTPase-activating protein TBC1D1 regulates GLUT4 translocation. J Biol Chem 284, 30016-23 19740738 Curated Info
31	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
32	Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26 19367708 Curated Info
33	Taylor EB, et al. (2008) Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle. J Biol Chem 283, 9787-96 18276596 Curated Info
34	Guo A (2007) CST Curation Set: 3496; Year: 2007; Biosample/Treatment: cell line, E1-1796/rapamycin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS/TF/Y) Immunoaffinity Beads Cat#: 1992 Curated Info
35	Guo A (2007) CST Curation Set: 3121; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS/TF/Y) Immunoaffinity Beads Cat#: 1992 Curated Info