Ser116
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Home > Phosphorylation Site Page: > Ser116  -  MEKK4 (mouse)

Site Information
KMNtPSQsPHKDLGK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 20804890

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , multiple reaction monitoring (MRM) ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 ) , 'muscle, skeletal' ( 1 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , RAW 264.7 (macrophage) ( 2 )

Upstream Regulation
Treatments:
insulin ( 3 ) , MK-2206 ( 3 )

Downstream Regulation
Effects of modification on MEKK4:
enzymatic activity, induced ( 1 ) , molecular association, regulation ( 1 )
Induce interaction with:
GADD45A (mouse) ( 1 )

References 

1

Bullard SA, et al. (2016) Gadd45a Protein Promotes Skeletal Muscle Atrophy by Forming a Complex with the Protein Kinase MEKK4. J Biol Chem 291, 17496-509
27358404   Curated Info

2

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

5

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info