Ser516
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Home > Phosphorylation Site Page: > Ser516  -  ARHGEF7 (rat)

Site Information
RKPERKPsDEEFAVR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455215

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 , 6 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 , 5 , 6 ) , phospho-antibody ( 5 ) , western blotting ( 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 2 ) , 'brain, cerebellum' ( 2 ) , 'brain, cerebral cortex' ( 2 ) , 293 (epithelial) ( 4 , 5 ) , adipose tissue ( 2 ) , HMC-1 ( 6 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 2 ) , liver ( 2 , 3 ) , lung ( 2 ) , neuron-'brain, hippocampus' ( 5 ) , spleen ( 2 ) , stomach ( 2 ) , testis ( 2 )

Upstream Regulation
Putative in vivo kinases:
CAMK1A (human) ( 5 ) , PKACA (human) ( 4 )
Kinases, in vitro:
CAMK1A (human) ( 5 ) , PKACA (human) ( 6 )
Treatments:
cAMP_analog ( 6 ) , colforsin ( 4 , 5 ) , depolarization ( 5 ) , endothelin ( 6 ) , phorbol_ester ( 5 ) , PKI ( 5 ) , serum ( 1 )

Downstream Regulation
Effects of modification on ARHGEF7:
intracellular localization ( 6 ) , molecular association, regulation ( 4 , 5 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 4 , 5 )
Induce interaction with:
14-3-3 beta (human) ( 4 ) , CDC42 iso1 (human) ( 5 ) , RAC1 (human) ( 5 )

References 

1

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Chahdi A, Sorokin A (2008) Protein kinase A-dependent phosphorylation modulates beta1Pix guanine nucleotide exchange factor activity through 14-3-3beta binding. Mol Cell Biol 28, 1679-87
18160719   Curated Info

5

Saneyoshi T, et al. (2008) Activity-dependent synaptogenesis: regulation by a CaM-kinase kinase/CaM-kinase I/betaPIX signaling complex. Neuron 57, 94-107
18184567   Curated Info

6

Chahdi A, Miller B, Sorokin A (2005) Endothelin 1 induces beta 1Pix translocation and Cdc42 activation via protein kinase A-dependent pathway. J Biol Chem 280, 578-84
15513924   Curated Info