Ser429

Site Information
ELIAGGGssCsPVLs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2020877

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 5 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 , 8 , 9 , 10 ) , mutation of modification site ( 5 , 8 ) , phospho-antibody ( 8 ) , western blotting ( 5 , 8 )
Disease tissue studied:	breast cancer ( 2 , 4 , 7 ) , breast ductal carcinoma ( 4 ) , breast cancer, triple negative ( 4 ) , ovarian cancer ( 4 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 3 , 8 , 9 ) , 293FT ( 5 ) , breast ( 4 ) , Jurkat (T lymphocyte) ( 10 ) , MCF-7 (breast cell) ( 2 ) , neuron-'brain, cerebral cortex' ( 5 ) , ovary ( 4 ) , SKBr3 (breast cell) ( 7 ) , Vero E6-S ('epithelial, kidney') ( 1 )

Downstream Regulation
Effects of modification on Huntingtin:	protein degradation ( 8 )

References
1	Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182 32645325 Curated Info
2	Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290 27362937 Curated Info
3	Huang B, et al. (2015) Scalable production in human cells and biochemical characterization of full-length normal and mutant huntingtin. PLoS One 10, e0121055 25799558 Curated Info
4	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
5	Watkin EE, et al. (2014) Phosphorylation of mutant huntingtin at serine 116 modulates neuronal toxicity. PLoS One 9, e88284 24505464 Curated Info
6	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
7	Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57 22964224 Curated Info
8	Dong G, et al. (2012) Mass spectrometric identification of novel posttranslational modification sites in Huntingtin. Proteomics 12, 2060-4 22623107 Curated Info
9	Mulhern D (2011) CST Curation Set: 12832; Year: 2011; Biosample/Treatment: cell line, HEK 293/ku-0063794; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS/T) Immunoaffinity Beads Cat#: 1979 Curated Info
10	Possemato A (2010) CST Curation Set: 10780; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (60D12F4) Rabbit mAb Cat#: 10002 Curated Info