Thr579

Site Information
EDEDVVLtPDGTREF SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2049877

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 )
Relevant cell line - cell type - tissue:	'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'brain, hippocampus, dentate gyrus' ( 4 ) , 'brain, midbrain' ( 4 ) , brain ( 2 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 1 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 1 ) , HL-1 (myocyte) ( 1 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 3 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 )

Relevant cell line - cell type - tissue:

'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'brain, hippocampus, dentate gyrus' ( 4 ) , 'brain, midbrain' ( 4 ) , brain ( 2 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 1 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 1 ) , HL-1 (myocyte) ( 1 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 3 )

References
1	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
2	Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29 22645316 Curated Info
3	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
4	Trinidad JC, et al. (2008) Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7, 684-96 18056256 Curated Info