Ser346
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Home > Phosphorylation Site Page: > Ser346  -  IRAK4 (human)

Site Information
FAQtVMtsRIVGTtA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2045338

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 3 , 5 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 2 , 5 ) , phospho-antibody ( 2 , 5 ) , western blotting ( 2 , 5 )
Relevant cell line - cell type - tissue:
fibroblast-skin [IRAK4 (human), homozygous knockout] ( 2 ) , HEK293T (epithelial) ( 5 ) , Jurkat (T lymphocyte) ( 3 ) , SF9 ( 6 )

Upstream Regulation
Putative in vivo kinases:
IRAK4 (human) ( 2 , 5 )
Kinases, in vitro:
IRAK4 (human) ( 2 , 5 )
Treatments:
compound_1 ( 2 ) , IL-1b ( 2 )

Downstream Regulation
Effects of modification on IRAK4:
enzymatic activity, induced ( 4 , 5 ) , protein conformation ( 6 )

References 

1

Ferrao R, et al. (2014) IRAK4 Dimerization and trans-Autophosphorylation Are Induced by Myddosome Assembly. Mol Cell 55, 891-903
25201411   Curated Info

2

Cushing L, et al. (2014) Interleukin 1/Toll-like receptor-induced autophosphorylation activates interleukin 1 receptor-associated kinase 4 and controls cytokine induction in a cell type-specific manner. J Biol Chem 289, 10865-75
24567333   Curated Info

3

Zhou J (2011) CST Curation Set: 12495; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Kuglstatter A, et al. (2007) Cutting Edge: IL-1 receptor-associated kinase 4 structures reveal novel features and multiple conformations. J Immunol 178, 2641-5
17312103   Curated Info

5

Cheng H, et al. (2007) Regulation of IRAK-4 kinase activity via autophosphorylation within its activation loop. Biochem Biophys Res Commun 352, 609-16
17141195   Curated Info

6

Wang Z, et al. (2006) Crystal structures of IRAK-4 kinase in complex with inhibitors: a serine/threonine kinase with tyrosine as a gatekeeper. Structure 14, 1835-44
17161373   Curated Info