Thr345
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Home > Phosphorylation Site Page: > Thr345  -  IRAK4 (human)

Site Information
kFAQtVMtsRIVGTt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2045325

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 2 , 6 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 3 , 6 ) , phospho-antibody ( 1 , 2 , 3 , 6 ) , western blotting ( 1 , 2 , 3 , 6 )
Relevant cell line - cell type - tissue:
fibroblast ( 1 ) , fibroblast-skin [IRAK4 (human), homozygous knockout] ( 3 ) , HEK293T (epithelial) ( 6 ) , SF9 ( 7 )

Upstream Regulation
Putative in vivo kinases:
IRAK4 (human) ( 3 , 6 )
Kinases, in vitro:
IRAK4 (human) ( 2 , 3 , 6 )
Treatments:
compound_1 ( 3 ) , IL-1b ( 3 )

Downstream Regulation
Effects of modification on IRAK4:
enzymatic activity, induced ( 2 , 5 , 6 ) , molecular association, regulation ( 2 ) , protein conformation ( 2 , 7 )
Inhibit interaction with:
IRAK4 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
pancreatic ductal adenocarcinoma ( 1 )

References 

1

Zhang D, et al. (2018) Tumor-stroma IL-1β-IRAK4 feedforward circuitry drives tumor fibrosis, chemoresistance, and poor prognosis in pancreatic cancer. Cancer Res
29363544   Curated Info

2

Ferrao R, et al. (2014) IRAK4 Dimerization and trans-Autophosphorylation Are Induced by Myddosome Assembly. Mol Cell 55, 891-903
25201411   Curated Info

3

Cushing L, et al. (2014) Interleukin 1/Toll-like receptor-induced autophosphorylation activates interleukin 1 receptor-associated kinase 4 and controls cytokine induction in a cell type-specific manner. J Biol Chem 289, 10865-75
24567333   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Kuglstatter A, et al. (2007) Cutting Edge: IL-1 receptor-associated kinase 4 structures reveal novel features and multiple conformations. J Immunol 178, 2641-5
17312103   Curated Info

6

Cheng H, et al. (2007) Regulation of IRAK-4 kinase activity via autophosphorylation within its activation loop. Biochem Biophys Res Commun 352, 609-16
17141195   Curated Info

7

Wang Z, et al. (2006) Crystal structures of IRAK-4 kinase in complex with inhibitors: a serine/threonine kinase with tyrosine as a gatekeeper. Structure 14, 1835-44
17161373   Curated Info