Ser260
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Home > Phosphorylation Site Page: > Ser260  -  MDM2 (human)

Site Information
sLDsEDYsLsEEGQE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454923

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , immunoprecipitation ( 1 , 3 ) , mass spectrometry ( 2 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 1 , 3 , 5 , 6 ) , phospho-antibody ( 3 , 5 , 6 ) , phosphopeptide mapping ( 6 ) , western blotting ( 1 , 3 , 5 , 6 )
Disease tissue studied:
lung cancer ( 1 , 3 , 5 , 6 ) , non-small cell lung cancer ( 1 , 3 , 5 , 6 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PLK1 (human) ( 3 )
Kinases, in vitro:
CK1D (human) ( 2 ) , CK2A1 (human) ( 4 ) , PLK1 (human) ( 3 )
Putative upstream phosphatases:
PPP2CA (human) ( 1 )
Treatments:
ionizing_radiation ( 6 ) , MG132 ( 2 )

Downstream Regulation
Effects of modification on MDM2:
activity, induced ( 6 ) , enzymatic activity, induced ( 3 ) , molecular association, regulation ( 1 ) , protein degradation ( 5 ) , ubiquitination ( 3 )
Inhibit interaction with:
Rb (human) ( 1 )

References 

1

Zheng Z, et al. (2012) 5-Aza-2'-deoxycytidine reactivates gene expression via degradation of pRb pocket proteins. FASEB J 26, 449-59
21990374   Curated Info

2

Inuzuka H, et al. (2010) Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase. Cancer Cell 18, 147-59
20708156   Curated Info

3

Dias SS, Hogan C, Ochocka AM, Meek DW (2009) Polo-like kinase-1 phosphorylates MDM2 at Ser260 and stimulates MDM2-mediated p53 turnover. FEBS Lett 583, 3543-8
19833129   Curated Info

4

Allende-Vega N, Dias S, Milne D, Meek D (2005) Phosphorylation of the acidic domain of Mdm2 by protein kinase CK2. Mol Cell Biochem 274, 85-90
16335531   Curated Info

5

Balass M, et al. (2002) Characterization of two peptide epitopes on Mdm2 oncoprotein that affect p53 degradation. Peptides 23, 1719-25
12383858   Curated Info

6

Blattner C, Hay T, Meek DW, Lane DP (2002) Hypophosphorylation of Mdm2 augments p53 stability. Mol Cell Biol 22, 6170-82
12167711   Curated Info