Tyr311

Site Information
TPETVGIyQGFEKKT SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447573
Associated spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 18 ) , electrophoretic mobility shift ( 25 ) , flow cytometry ( 28 ) , mass spectrometry ( 2 , 3 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 19 , 20 , 21 , 22 , 23 , 27 ) , mutation of modification site ( 7 , 15 , 17 , 18 , 24 , 26 , 27 , 28 ) , phospho-antibody ( 1 , 4 , 7 , 15 , 16 , 17 , 18 , 25 , 26 , 27 ) , western blotting ( 1 , 4 , 7 , 15 , 16 , 17 , 25 , 26 , 27 , 28 )
Disease tissue studied:	adrenal cancer ( 9 , 10 ) , pheochromocytoma ( 9 , 10 ) , neuroblastoma ( 4 )
Relevant cell line - cell type - tissue:	'kidney, cortex' ( 3 ) , 293 (epithelial) ( 17 , 28 ) , 3T3 (fibroblast) ( 28 ) , brain ( 5 , 6 , 8 , 19 , 20 , 21 , 22 , 23 ) , cardiac ( 16 ) , COS (fibroblast) ( 15 , 27 ) , HEK293T (epithelial) [VEGFR2 (human)] ( 24 ) , HeLa (cervical) ( 26 ) , intestine ( 2 ) , lung ( 2 ) , MEF (fibroblast) [PKCE (mouse)] ( 16 ) , myocyte-heart ( 7 , 25 ) , N27 (neuron) ( 18 ) , Neuro-2a (neuron) ( 4 ) , ParC5 ( 1 ) , PC-12 (chromaffin) ( 9 , 10 , 11 , 12 , 13 , 14 ) , stomach ( 2 ) , SYF (fibroblast) ( 16 )

Upstream Regulation
Regulatory protein:	PKCE (mouse) ( 16 )
Putative in vivo kinases:	Src (rat) ( 1 , 18 , 25 , 28 )
Kinases, in vitro:	EGFR (human) ( 15 ) , Lck (mouse) ( 27 ) , Src (human) ( 7 , 17 ) , Src (rat) ( 16 , 25 )
Treatments:	AG1478 ( 15 ) , ceramide ( 26 ) , curcumin ( 4 ) , dasatinib ( 1 ) , EGF ( 15 ) , GF109203X ( 17 ) , H2O2 ( 1 , 7 , 15 , 16 , 17 , 18 , 25 , 27 ) , herbimycin_A ( 26 ) , imatinib ( 1 ) , mutation ( 1 ) , PD153035 ( 15 ) , peptide inhibitor ( 18 ) , phorbol_ester ( 16 , 17 , 25 ) , PP1 ( 7 , 25 ) , PP2 ( 26 ) , siRNA ( 1 ) , vanadate ( 8 )

Downstream Regulation
Effects of modification on PKCD:	enzymatic activity, induced ( 7 , 18 , 26 , 27 ) , protein degradation ( 4 , 18 , 28 )
Effects of modification on biological processes:	apoptosis, induced ( 18 , 26 )

References
1	Wie SM, et al. (2014) Inhibiting tyrosine phosphorylation of protein kinase Cδ (PKCδ) protects the salivary gland from radiation damage. J Biol Chem 289, 10900-8 24569990 Curated Info
2	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
3	Feric M, et al. (2011) Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule. Am J Physiol Cell Physiol 300, C755-70 21209370 Curated Info
4	Conboy L, et al. (2009) Curcumin-induced degradation of PKC delta is associated with enhanced dentate NCAM PSA expression and spatial learning in adult and aged Wistar rats. Biochem Pharmacol 77, 1254-65 19161989 Curated Info
5	Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
6	Guo A (2008) CST Curation Set: 5222; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
7	Sumandea MP, et al. (2008) Tyrosine phosphorylation modifies protein kinase C delta-dependent phosphorylation of cardiac troponin I. J Biol Chem 283, 22680-9 18550549 Curated Info
8	Lewandrowski U, et al. (2008) Identification of new tyrosine phosphorylated proteins in rat brain mitochondria. FEBS Lett 582, 1104-10 18331841 Curated Info
9	Stokes M (2008) CST Curation Set: 4008; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
10	Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	Guo A (2008) CST Curation Set: 3877; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	Guo A (2008) CST Curation Set: 3878; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
13	Guo A (2008) CST Curation Set: 3879; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	Guo A (2008) CST Curation Set: 3880; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	Morita M, et al. (2008) Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. J Biochem 143, 31-8 17956904 Curated Info
16	Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8 17569658 Curated Info
17	Rybin VO, Steinberg SF (2006) Immunoblotting PKC-delta: a cautionary note from the bench. Am J Physiol Cell Physiol 290, C750-6 16236821 Curated Info
18	Kaul S, et al. (2005) Tyrosine phosphorylation regulates the proteolytic activation of protein kinase Cdelta in dopaminergic neuronal cells. J Biol Chem 280, 28721-30 15961393 Curated Info
19	Guo A (2005) CST Curation Set: 678; Year: 2005; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
20	Guo A (2005) CST Curation Set: 679; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 15'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
21	Guo A (2005) CST Curation Set: 680; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
22	Guo A (2005) CST Curation Set: 532; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
23	Guo A (2005) CST Curation Set: 522; Year: 2005; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
24	Kuriyama M, et al. (2004) Activation and translocation of PKCdelta is necessary for VEGF-induced ERK activation through KDR in HEK293T cells. Biochem Biophys Res Commun 325, 843-51 15541367 Curated Info
25	Rybin VO, et al. (2004) Stimulus-specific differences in protein kinase C delta localization and activation mechanisms in cardiomyocytes. J Biol Chem 279, 19350-61 14970215 Curated Info
26	Kajimoto T, et al. (2004) Ceramide-induced apoptosis by translocation, phosphorylation, and activation of protein kinase Cdelta in the Golgi complex. J Biol Chem 279, 12668-76 14715667 Curated Info
27	Konishi H, et al. (2001) Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro. Proc Natl Acad Sci U S A 98, 6587-92 11381116 Curated Info
28	Blake RA, Garcia-Paramio P, Parker PJ, Courtneidge SA (1999) Src promotes PKCdelta degradation. Cell Growth Differ 10, 231-41 10319993 Curated Info