Tyr311
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Home > Phosphorylation Site Page: > Tyr311  -  PKCD (rat)

Site Information
TPETVGIyQGFEKKT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447573
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 18 ) , electrophoretic mobility shift ( 25 ) , flow cytometry ( 28 ) , mass spectrometry ( 2 , 3 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 19 , 20 , 21 , 22 , 23 , 27 ) , mutation of modification site ( 7 , 15 , 17 , 18 , 24 , 26 , 27 , 28 ) , phospho-antibody ( 4 , 7 , 15 , 16 , 17 , 18 , 25 , 26 , 27 ) , western blotting ( 4 , 7 , 15 , 16 , 17 , 25 , 26 , 27 , 28 )
Disease tissue studied:
adrenal cancer ( 9 , 10 ) , pheochromocytoma ( 9 , 10 ) , neuroblastoma ( 4 )
Relevant cell line - cell type - tissue:
'kidney, cortex' ( 3 ) , 293 (epithelial) ( 17 , 28 ) , 293T (epithelial) [VEGFR2 (human)] ( 24 ) , 3T3 (fibroblast) ( 28 ) , brain ( 5 , 6 , 8 , 19 , 20 , 21 , 22 , 23 ) , cardiac ( 16 ) , COS (fibroblast) ( 15 , 27 ) , HeLa (cervical) ( 26 ) , intestine ( 2 ) , lung ( 2 ) , MEF (fibroblast) [PKCE (mouse)] ( 16 ) , myocyte-heart ( 7 , 25 ) , N27 (neuron) ( 18 ) , Neuro-2a (neuron) ( 4 ) , PC-12 (chromaffin) ( 9 , 10 , 11 , 12 , 13 , 14 ) , stomach ( 2 ) , SYF (fibroblast) ( 16 )

Upstream Regulation
Regulatory protein:
PKCE (mouse) ( 16 )
Putative in vivo kinases:
Src (rat) ( 18 , 25 , 28 )
Kinases, in vitro:
EGFR (human) ( 15 ) , Lck (mouse) ( 27 ) , Src (human) ( 7 , 17 ) , Src (rat) ( 16 , 25 )
Treatments:
AG1478 ( 15 ) , ceramide ( 26 ) , curcumin ( 4 ) , EGF ( 15 ) , GF109203X ( 17 ) , H2O2 ( 7 , 15 , 16 , 17 , 18 , 25 , 27 ) , herbimycin_A ( 26 ) , PD153035 ( 15 ) , peptide inhibitor ( 18 ) , phorbol_ester ( 16 , 17 , 25 ) , PP1 ( 7 , 25 ) , PP2 ( 26 ) , vanadate ( 8 )

Downstream Regulation
Effects of modification on PKCD:
enzymatic activity, induced ( 7 , 18 , 26 , 27 ) , protein degradation ( 4 , 18 , 28 )
Effects of modification on biological processes:
apoptosis, induced ( 18 , 26 )

References 

1

Wie SM, et al. (2014) Inhibiting tyrosine phosphorylation of protein kinase Cδ (PKCδ) protects the salivary gland from radiation damage. J Biol Chem 289, 10900-8
24569990   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Feric M, et al. (2011) Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule. Am J Physiol Cell Physiol 300, C755-70
21209370   Curated Info

4

Conboy L, et al. (2009) Curcumin-induced degradation of PKC delta is associated with enhanced dentate NCAM PSA expression and spatial learning in adult and aged Wistar rats. Biochem Pharmacol 77, 1254-65
19161989   Curated Info

5

Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Guo A (2008) CST Curation Set: 5222; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Sumandea MP, et al. (2008) Tyrosine phosphorylation modifies protein kinase C delta-dependent phosphorylation of cardiac troponin I. J Biol Chem 283, 22680-9
18550549   Curated Info

8

Lewandrowski U, et al. (2008) Identification of new tyrosine phosphorylated proteins in rat brain mitochondria. FEBS Lett 582, 1104-10
18331841   Curated Info

9

Stokes M (2008) CST Curation Set: 4008; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Guo A (2008) CST Curation Set: 3877; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Guo A (2008) CST Curation Set: 3878; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Guo A (2008) CST Curation Set: 3879; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Guo A (2008) CST Curation Set: 3880; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Morita M, et al. (2008) Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. J Biochem 143, 31-8
17956904   Curated Info

16

Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8
17569658   Curated Info

17

Rybin VO, Steinberg SF (2006) Immunoblotting PKC-delta: a cautionary note from the bench. Am J Physiol Cell Physiol 290, C750-6
16236821   Curated Info

18

Kaul S, et al. (2005) Tyrosine phosphorylation regulates the proteolytic activation of protein kinase Cdelta in dopaminergic neuronal cells. J Biol Chem 280, 28721-30
15961393   Curated Info

19

Guo A (2005) CST Curation Set: 678; Year: 2005; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

20

Guo A (2005) CST Curation Set: 679; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 15'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

21

Guo A (2005) CST Curation Set: 680; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

22

Guo A (2005) CST Curation Set: 532; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

23

Guo A (2005) CST Curation Set: 522; Year: 2005; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

24

Kuriyama M, et al. (2004) Activation and translocation of PKCdelta is necessary for VEGF-induced ERK activation through KDR in HEK293T cells. Biochem Biophys Res Commun 325, 843-51
15541367   Curated Info

25

Rybin VO, et al. (2004) Stimulus-specific differences in protein kinase C delta localization and activation mechanisms in cardiomyocytes. J Biol Chem 279, 19350-61
14970215   Curated Info

26

Kajimoto T, et al. (2004) Ceramide-induced apoptosis by translocation, phosphorylation, and activation of protein kinase Cdelta in the Golgi complex. J Biol Chem 279, 12668-76
14715667   Curated Info

27

Konishi H, et al. (2001) Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro. Proc Natl Acad Sci U S A 98, 6587-92
11381116   Curated Info

28

Blake RA, Garcia-Paramio P, Parker PJ, Courtneidge SA (1999) Src promotes PKCdelta degradation. Cell Growth Differ 10, 231-41
10319993   Curated Info